Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1979-04-09
1981-03-17
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
23230B, 424 8, 424 12, 435 5, 435810, C12Q 166, A61K 3700, G01N 3100, G01N 2300
Patent
active
042568340
ABSTRACT:
Novel immunoassays are provided employing discrete particulate reagents for determining an analyte which is a member of a specific binding pair-ligand and homologous receptor. The assay employs as a first reagent, a member of said pair bound to an insoluble particle (particle conjugate); as a second reagent, a label which is part of a signal producing system, bound to a member of said pair (signal label conjugate); and as a third reagent, a signal repressor comprising an insoluble particle, where the signal repressor is obstructed from interacting with said label of said signal label conjugate, when said signal label conjugate is bound to said particle conjugate.
In performing the assay, the analyte, the reagents, and any ancillary materials are combined in an aqueous assay medium and the signal determined as compared to an assay medium having a known amount of analyte.
The repressor greatly enhances the sensitivity and accuracy of the immunoassay in repressing the signal produced by labels which are not bound to the particle conjugate, thus substantially limiting the observed signal to label bound to the particle conjugate. The labels which are employed provide a signal which does not differ significantly from when the signal label conjugate is bound to the particle conjugate or is free in the bulk solution. Illustrative labels include chromogens, such as fluorescers, chemiluminescers, and the like.
Particular reagents and kits are provided, where the kits have predetermined amounts of the various reagents to substantially optimize the sensitivity of the assay.
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patent: 4108972 (1978-08-01), Dreyer
patent: 4115535 (1978-09-01), Giaever
patent: 4130462 (1978-12-01), Rubenstein et al.
patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4160645 (1979-07-01), Ullman
patent: 4161515 (1979-07-01), Ullman
patent: 4166104 (1979-08-01), Wagner et al.
patent: 4193983 (1980-03-01), Ullman et al.
Ullman Edwin F.
Zuk Robert F.
Rowland Bertram I.
Syva Company
Wiseman Thomas G.
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