Fluorescent reporter beads for fluid analysis

Details Fluorescent reporter beads for fluid analysis Fluorescent reporter beads for fluid analysis

1996-03-20

1998-05-05

Soderquist, Arlen

Chemistry: analytical and immunological testing

Optical result

With fluorescence or luminescence

356246, 422 81, 422 8205, 422 8208, 422 8209, 436 8, 436 10, 436 43, 436 52, 436 63, 436 68, 436 73, 436 79, 436 80, 436 81, 436 82, 436 83, 436 84, 436 86, 436 87, 436 91, 436100, 436103, 436127, 436136, G01N 3300, G01N 2101, G01N 2164

Patent

active

057473498

ABSTRACT:
The present invention provides a method and apparatus for rapid measurement of a fluid bulk analyte, requiring only microscale volumes. Several fluid bulk analytes can be measured simultaneously and, for biological samples, the cell content can also be measured simultaneously. The invention comprises reporter beads for chemical analysis of fluid bulk properties such as pH, oxygen saturation and ion content. Each reporter bead comprises a substrate bead having a plurality of at least one type of fluorescent reporter molecules immobilized thereon. The fluorescent properties of the reporter bead are sensitive to a corresponding analyte. Reporter beads are added to a fluid sample and the analyte concentration is determined by measuring fluorescence of individual beads, for example in a flow cytometer. Alternatively, reporter molecules which change absorbance as a function of analyte concentration can be employed.

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