Fluorescent proteins from Fungia

Details Fluorescent proteins from Fungia Fluorescent proteins from Fungia

2007-04-24

2009-06-02

Noakes, Suzanne M. (Department: 1656)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C530S350000, C530S400000

Reexamination Certificate

active

07541451

ABSTRACT:
An object of the present invention is to provide a novel fluorescent protein derived from organisms other thanAequorea victoria. According to the present invention, there is provided a fluorescent protein derived fromFungiasp., which has the following properties:(1) the excitation maximum wavelength is 455 nm, and the fluorescence maximum wavelength is 488 nm;(2) the molar absorption coefficient at 455 nm is 38700 or 27700;(3) the quantum yield is 0.85 or 0.81; and(4) the pH sensitivity of the fluorescent property is stable at pH 5 to 9; anda fluorescent protein derived fromFungiasp., which has the following properties:(1) the excitation maximum wavelength is 548 nm, and the fluorescence maximum wavelength is 561 nm;(2) the molar absorption coefficient at 548 nm is 75900 or 51000;(3) the quantum yield is 0.44 or 0.50; and(4) the pH sensitivity of the fluorescent property is pKa<5.0.

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