Fluorescent proteins as cell-type specific reporters

Details Fluorescent proteins as cell-type specific reporters Fluorescent proteins as cell-type specific reporters

2006-09-12

2006-09-12

Woitach, Joseph T. (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C536S023100, C536S023400

Reexamination Certificate

active

07105344

ABSTRACT:
The present invention related to non-human mammal embryonic stem (ES) cells stably transfected with a DNA construct comprising a DNA sequence coding for a non-cell damaging fluorescent protein and a cell- and/or development-dependent promoter operably linked with said DNA sequence; a method for preparing such ES cells; a cell culture obtainable by culturing said ES cells; a method for the toxicological examination of substances using such cell cultures; a method for producing transgenic non-human mammals using said ES cells; a transgenic non-human mammal obtainable by said method; and a method for examining stages of cellular development using cells of such a non-human mammal.

REFERENCES:
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