Fluorescent proteins and methods of using same

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S069100, C435S007100, C530S350000, C514S002600

Reexamination Certificate

active

11251209

ABSTRACT:
The present invention provides novel engineered derivatives of green fluorescent protein (GFP) which have an amino acid sequence which is modified by amino acid substitution compared with the amino acid sequence of wild type Green Fluorescent Protein (SEQ ID NO: 2). The modified GFPs exhibit enhanced fluorescence relative to wtGFP when expressed in non-homologous cells at temperatures above 30° C., and when excited at about 490 nm compared to the parent proteins, i.e. wtGFP. An example of a preferred protein is F64L-S175G-E222G-GFP. The modified GFPs provide a means for detecting GFP reporters in mammalian cells at lower levels of expression and/or increased sensitivity relative to wtGFP. This greatly improves the usefulness of fluorescent proteins in studying cellular functions in living cells.

REFERENCES:
patent: 5491084 (1996-02-01), Chalfie et al.
patent: 5804387 (1998-09-01), Cormack et al.
patent: 6027881 (2000-02-01), Pavlakis et al.
patent: 6054321 (2000-04-01), Tsien et al.
patent: 6077707 (2000-06-01), Tsien et al.
patent: 6150176 (2000-11-01), Tsien et al.
patent: 6172188 (2001-01-01), Thastrup et al.
patent: 6194548 (2001-02-01), Osumi et al.
patent: 6780975 (2004-08-01), Tsien et al.
patent: WO 96/27675 (1996-09-01), None
patent: WO 97/42320 (1997-11-01), None
patent: WO 00/08054 (2000-02-01), None
patent: WO 01/98338 (2001-12-01), None
Brejc, K., et al., “Structural basis for dual excitation and photoisomerization of theAequorea victoriagreen fluorescent protein”,Proc. Natl. Acad. Sci., vol. 94, 1997, p. 2306-2311.
Crameri, A., et al., “Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling”,Nature Biotechnology, vol. 14, 1996, p. 315-319.
Chalfie, M., et al., “Green Fluorescent Protein as a Marker for Gene Expression”,Science, vol. 263, 1994, p. 802-805.
Chalfie, M., “Green Fluorescent Protein”,Photochemistry and Photobiology, vol. 62, No. 4, 1995, pp. 651-656.
Cormack, B., et al., “FACS-optimized mutants of the green fluorescent protein (GFP)”,Gene, vol. 173, 1996, p. 33-38.
Ehrig, T., et al., “Green-fluorescent protein mutants with altered fluorescence excitation spectra”,Federation of the European Biochemical Societies Letters, vol. 367, 1995, p. 163-166.
Heim, R., et al., “Improved green fluorescence”,Nature, vol. 373, 1995, p. 663-664.
Heim, R., et al., “Wavelength mutations and posttranslational autoxidation of green fluorescent protein”,Proc. Natl. Acad. Sci., vol. 91, 1994, p. 12501-12504.
Reichel, C., et al., “Enhanced green fluorescence by the expression of anAequorea victoriagreen fluorescent protein mutant in mono- and dicotyledonous plant cells”,Proc. Natl. Acad. Sci., vol. 93, 1996, p. 5888-5893.
Yang, T., et al., “Improved Fluorescence and Dual Color Detection with Enhanced Blue and Green Variants of the Green Fluorescent Protein”,The Journal of Biological Chemistry, vol. 273, No. 14, 1998, p. 8212-8216.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Fluorescent proteins and methods of using same does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescent proteins and methods of using same, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescent proteins and methods of using same will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3843215

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.