Fluorescent proteins

Details Fluorescent proteins Fluorescent proteins

2006-02-21

2006-02-21

Wax, Robert A. (Department: 1653)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C530S300000, C435S069100, C435S007100, C435S320100, C435S252000, C514S002600, C514S012200, C536S023100

Reexamination Certificate

active

07001986

ABSTRACT:
A GFP with an F64L mutation and an E222G mutation is provided. This GFP has a bigger Stokes shift compared to other GFPs making it very suitable for high throughput screening due to a better resolution. This GFP also has an excitation maximum between the yellow GFP and the cyan GFP allowing for cleaner band separation when used together with those GFPs.

REFERENCES:
patent: 6124128 (2000-09-01), Tsien et al.
patent: 6172188 (2001-01-01), Thastrup et al.
patent: WO 97/11094 (1997-03-01), None
Roger Helm et al., Proc. Natl. Acad. Sci. USA, vol. 9, pp. 12501-12504, Dec. 1994.
Torsten Ehrig et al., FEBS Letters, 367 (1995) pp. 163-166.
Katjusa Brejc et al., Proc. Natl. Acad. Sci. USA, vol. 94, pp. 2306-2311, Mar. 1997.
Te-Tuan Yang et al., Nucleic Acids Research, 1996, vol. 24, No. 22, pp. 4592-4593.

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