Fluorescent protein sensors for detection of analytes

Details Fluorescent protein sensors for detection of analytes Fluorescent protein sensors for detection of analytes

1997-03-14

1999-12-07

Brusca, John S.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

4352523, 4352542, 4353201, 536 234, 536 235, C12N 510, C12N 1562, C12N 1512, C12N 1563

Patent

active

059982047

ABSTRACT:
Fluorescent indicators including a binding protein moiety, a donor fluorescent protein moiety, and an acceptor fluorescent protein moiety are described. The binding protein moiety has an analyte-binding region which binds an analyte and causes the indicator to change conformation upon exposure to the analyte. The donor moiety and the acceptor moiety change position relative to each other when the analyte binds to the analyte-binding region. The donor moiety and the acceptor moiety exhibit fluorescence resonance energy transfer when the donor moiety is excited and the distance between the donor moiety and the acceptor moiety is small. The indicators can be used to measure analyte concentrations in samples, such as calcium ion concentrations in cells.

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