Fluorescent protein

Details Fluorescent protein Fluorescent protein

2007-06-05

2007-06-05

Carlson, Karen Cochrane (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C435S069100

Reexamination Certificate

active

10498505

ABSTRACT:
An object of the present invention is to provide a novel fluorescent protein derived from organisms other thanAequorea victoria. According to the present invention, there is provided a fluorescent protein derived fromFungiasp., which has the following properties:(1) the excitation maximum wavelength is 455 nm, and the fluorescence maximum wavelength is 488 nm;(2) the molar absorption coefficient at 455 nm is 38700 or 27700;(3) the quantum yield is 0.85 or 0.81; and(4) the pH sensitivity of the fluorescent property is stable at pH 5 to 9; anda fluorescent protein derived fromFungiasp., which has the following properties:(1) the excitation maximum wavelength is 548 nm, and the fluorescence maximum wavelength is 561 nm;(2) the molar absorption coefficient at 548 nm is 75900 or 51000;(3) the quantum yield is 0.44 or 0.50; and(4) the pH sensitivity of the fluorescent property is pKa<5.0.

REFERENCES:
patent: 2003/0017538 (2003-01-01), Miyawaki et al.
patent: 00/34320 (2000-06-01), None
patent: 00/34526 (2000-06-01), None
patent: 01/27150 (2001-04-01), None
patent: 03/033693 (2003-04-01), None
patent: 03/070952 (2003-08-01), None
A. Salih et al., Nature, vol. 408, No. 6814, Dec. 14, 2000, pp. 850-853.
N. Gurskaya et al., BMC Biochemistry, vol. 2, No. 6, Jul. 10, 2001, pp. 1-7.
U.S. Appl. No. 10/581,551 to Miyawaki, filed Jun. 2, 2006.
U.S. Appl. No. 11/390,215 to Miyawaki, filed Mar. 28, 2006.
R.Y. Tsien, Ann. Rev. Biochem., vol. 67, pp. 509-544, 1998.
M.V. Matz et al., Nat. Biotechnol., vol. 17, No. 10, pp. 969-973, 1999.
K.A. Lukyanov et al., J. Biol. Chem., vol. 275, No. 34, pp. 25879-25882, 2000.
T. Hosaka, Kagaku to Kogyo, vol. 53, No. 5, pp. 612, 2000.
A.F. Fradkov et al., FEBS Letters, vol. 479, No. 3, pp. 127-130, 2000.
A.A. Heikal et al., Proc. Natl. Acad. Sci. USA, vol. 97, No. 22, pp. 11996-12001, 2000.
Oz Reef Press. Resident of the Month, Oz Reef Marine Park, Jun. 1998, retrieved on Mar. 3, 2003 from http://ozreef.org/press/1998/june.html.
Oz Reef Press. Resident of the Month, Oz Reef Marine Park, May 1997, retrieved on Mar. 3, 2003 from http://ozreef.org/press/1997/may.html.

Affiliated with

Also associated with

Rating

Fluorescent protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescent protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescent protein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3827331