Fluorescent protein

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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Details

C435S320100, C435S252200, C536S023100, C530S350000

Reexamination Certificate

active

10492081

ABSTRACT:
An object of the present invention is to provide a novel fluorescent protein derived from organisms other thanAequorea victoria. According to the present invention, there is provided a fluorescent protein derived fromGalaxea fascicularis, which has the following properties:(1) the molecular weight is approximately 27,000;(2) a tetramer is formed in an equilibration state;(3) the excitation maximum wavelength is 492 nm, and the fluorescence maximum wavelength is 505 nm;(4) the molar absorption coefficient is 74,100;(5) the quantum yield is 0.625; and(6) the pH sensitivity of the fluorescent property is low in the range between pH 5 and pH 12.

REFERENCES:
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patent: 2006/0154296 (2006-07-01), Miyawaki et al.
patent: 2006/0160990 (2006-07-01), Miyawaki et al.
patent: 2006/0240472 (2006-10-01), Miyawaki et al.
patent: 10-234382 (1998-09-01), None
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R.Y. Tsien, Ann. Rev. Biochem., vol. 67, pp. 509-544, 1998.
English Language abstract of JP 10-234382.
Geoffrey S. Baird et al., “Biochemistry, Mutagenesis, and Oligomerization of DsRed, a Red Fluorescent Protein From Coral”, PNAS, vol. 97, No. 22, pp. 11984-11989 (2000).
U.S. Appl. No. 11/390,215 (Miyawaki et al.), filed Mar. 28, 2006 and entitled “A Method for Analysis of Protein Interaction using Fluorescent Protein”.
U.S. Appl. No. 10/525,365 (Miyawaki et al.), filed Aug. 22, 2003 and entitled “Fluorescent protein and Chromoprotein”.
U.S. Appl. No. 10/581,551 (Miyawaki et al.), filed Dec. 3, 2004 and entitled “Fluorescent Proteins”.
U.S. Appl. No. 10/561,041 (Miyawaki et al.), filed Jun. 16, 2004 and entitled “Fluorescent Protein and Pigment Protein”.
U.S. Appl. No. 10/561,040 (Miyawaki et al.), filed Jun. 16, 2004 and entitled “Fluorescent Protein”.
U.S. Appl. No. 10/516,314 (Miyawaki et al.), filed Jun. 10, 2003 and entitled “Pigment Protein”.
U.S. Appl. No. 10/516,317 (Miyawaki et al.), filed Jun. 10, 2003 and entitled “Pigment Protein”.

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