Fluorescent protein

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

Reexamination Certificate

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Details

C435S252300, C435S254100, C435S410000, C530S350000, C536S023100

Reexamination Certificate

active

07897385

ABSTRACT:
The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b);(a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or(b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.

REFERENCES:
patent: 6046925 (2000-04-01), Tsien et al.
patent: 2003/0092884 (2003-05-01), Lukyanov et al.
patent: 2003/0157643 (2003-08-01), Almond et al.
patent: 2005/0032085 (2005-02-01), Labas et al.
patent: 98/47148 (1998-10-01), None
patent: 02/096924 (2002-12-01), None
patent: 03/042401 (2003-05-01), None
Supplementary material of seven pages for the IDS reference Ando et al. Sience Nov. 19, 2004, vol. 306, pp. 1370-1373.
Habuchi et al., “Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, No. 27, Jul. 2005, pp. 9511-9516, XP002476316, ISSN: 0027-8424.
Kelmanson IV et al., Mol. Biol. Evol., vol. 20, No. 7, (2003), pp. 1125-1133.
Lesser, M. et al., The DDBJ/GenBank/EMBL databases [online], Jul. 20, 2001, Accession No. AF401282.
Matz, M. et al., The DDBJ/GenBank/EMBL databases [online], May 30, 2001, Accession No. AY037766.
Ando et al., “Regulated Fast Nucleocytoplasmic Shuttling Observed by Reversible Protein Highlighting.” Science, vol. 306, No. 5700, Nov. 19, 2004, pp. 1370-1373.
Tsien, R.Y. et al., Ann. Rev. Biochem., 67, (1998), pp. 509-544.
Miyawaki, Cell Structure and Function, vol. 27, No. 5 (2002) pp. 343-347.
Labas et al., Proc. Nat. Acad. Sci. USA (PNAS), vol. 99, No. 7 (2002) pp. 4256-4261.
Japanese Office Action mailed Nov. 16, 2010 with partial English translation.

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