Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2011-03-01
2011-03-01
Nashed, Nashaat T (Department: 1656)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S252300, C435S254100, C435S410000, C530S350000, C536S023100
Reexamination Certificate
active
07897385
ABSTRACT:
The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b);(a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or(b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.
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Supplementary material of seven pages for the IDS reference Ando et al. Sience Nov. 19, 2004, vol. 306, pp. 1370-1373.
Habuchi et al., “Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, No. 27, Jul. 2005, pp. 9511-9516, XP002476316, ISSN: 0027-8424.
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Japanese Office Action mailed Nov. 16, 2010 with partial English translation.
Ando Ryoko
Karasawa Satoshi
Miyawaki Atsushi
Mizuno Hideaki
Nashed Nashaat T
Riken
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