Fluorescent probe for rapid measurement of analyte concentration

Details Fluorescent probe for rapid measurement of analyte concentration Fluorescent probe for rapid measurement of analyte concentration

1985-08-27

1991-07-23

Warden, Robert J.

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Chemiluminescent

422681, 422 55, 422 56, 422 57, 422 58, 422 52, 422 60, 435817, 436 95, 436172, 436163, G01N 3300

Patent

active

050341898

ABSTRACT:
A fluorescent optical probe employed for the analysis of the concentration of an analyte (or material to be analyzed) in a fluid, employs a permeable, transparent polymeric matrix in which a fluorophor is immobilized, with the polymeric material being directly exposed to the fluid being analyzed. The composite material of the probe may be made up of a homogeneous matrix of transparent polymer, fluorescent compound, catalyst(s) and reagents(s) and is employed to measure analyte concentration in a fluid in the environment surrounding the material. For analyzing oxygen, the fluorophor may be 9,10-diphenyl anthracene and the polymer matrix may be poly (dimethyl siloxane), or silicone, with the presence of oxygen quenching the fluorescence of 9,10-diphenyl anthracene. For analyzing the concentration of glucose, the polymeric material may be poly (hydroxyethyl methacrylate), or PHEMA, the fluorophor may be 9,10-diphenyl anthracene, and as catalytic material, glucose oxidase may also be immobilized within the PHEMA matrix to reduce the quenching action of the oxygen, with increased output radiation therefore indicating higher levels of glucose. More generally, the fluorophor, and the catalyst, and other reagents, when utilized, are immobilized, either physically or chemically, in a homogeneous manner throughtout the polymer host. Examples of other analytes and catalysts are given.

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Cox et al., Applied Optics, vol. 24(14), Jul. 15, 1985.
CA 76:39547c, Johnson et al.
CA 74:69921w, Tolstorozhev et al.
Bowen et al., J of Phy. Chem., 1959 (63), pp. 4-7.

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