Fluorescent polypeptide complex

Details Fluorescent polypeptide complex Fluorescent polypeptide complex

2006-10-05

2010-02-16

Desai, Anand U (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C977S705000, C514S002600

Reexamination Certificate

active

07662920

ABSTRACT:
The present invention relates to a method of producing a fluorescent polypeptide complex characterized in that an isolated fluorescent polypeptide is inter-molecularly cross-linked with itself or one or more other polypeptides and wherein said complex is of 40 nm to 500 nm in size, it also relates to a complex obtainable by such method, to conjugates comprising such a fluorescent polypeptide complex and to the use of such complex or conjugate.

REFERENCES:
patent: 4169137 (1979-09-01), Hirschfeld et al.
patent: 4542104 (1985-09-01), Stryer et al.
patent: 5516635 (1996-05-01), Ekins et al.
patent: 5661040 (1997-08-01), Huff et al.
patent: 5891741 (1999-04-01), Siiman et al.
patent: 0821742 (1998-02-01), None
patent: 58222029 (1983-12-01), None
patent: WO 93/03062 (1993-02-01), None
patent: WO 98/36099 (1998-08-01), None
patent: WO 01/96383 (2001-12-01), None
Rumbeli et al.,“Crosslinking of Phycobiliproteins from the Cyanobacterium Mastigocladus laminosus with Bis-Imidates: Localization of an Intrasubuint and an Intersubunit Crosslink in C-Phycocyanin”, Biol. Chem. vol. 368, pp. 1179-1191 Sep. 1987.
Allen, J. et al., “Finding Prospective Partners in the Library: the Two-Hybrid System and Phage Display Find a Match,” TIBS 20, Dec. 1995, 511-516.
Custer, M. et al., “A Biologic Assay for IL-4,” Jounal of Immunological Methods, 128(1990) 109-117.
Dent, Al. et al., “The Preparation of Protein-Protein Conjugates,” Bioconjugation (1988) 216-363.
Gantt, E., “Phycobilisomes: Light-Harvesting Pigment Complexes,” BioScience vol. 25, No. 12, Dec. 1975, 781-788.
Glazer, A. et al., “Fluorescent Tandem Pycobiliprotein Conjugates, Emission Wavelength Shifting by Energy Transfer,” Biophys. J., vol. 43, Sep. 1983, 386-386.
Kronick, M. et al., “Immunoassay Techniques with Fluorescent Pycobiliprotein Conjugates,” Clin. Chem. 29/9, 1582-1586 (1983).
Ong, L. et al., “Crosslinking of allophycocyanin,” Physiol. Veg., 1985, 23(1) 777-787.
Papageorgiou, G. et al., “Effects of Chaotropic Electrolytes on the Structure and Electronic Excitation Coupling of Glutaraldehyde-and Diimido Ester-Cross-Linked Phycobilisomes,” Biochimica et Biophysica Acta, 724(1983) 323-332.
Schechter, Y, et al., “Fluorescent labeling of hormone receptors in viable cells: Preparation and properties of highly fluorescent derivatives of epidermal growth factor and insulin,” Proc. Natl. Acad. Sci., vol. 75, No. 5, 2135-2319, May 1978.
Siiman, O. et al., “Fluorescent Neoglycoproteins: Antibody-Aminodextran-Phycobiliprotein Conjugates,” Bioconjugate Chem. 1999, 10, 1090-1106.
Telford, W. et al., “Cyanobacterial stabilized phycobilisomes as fluorochromes for extracellular antigen detection by flow cytometry,” Journal of Immunological Methods, 254(2001) 13-30.
Trinquet, E. et al., “Allophycocyanin 1 as a Near-Infared Fluorescent Tracer: Isolation, Characterization, Chemical Modification, and Use in a Homogeneous Fluorescence Resonance Energy Transfer System,” Analytical Biochemistry 296, 232-244 (2001).
Tijssen, P., Practice and Theory of Enzyme Immunoassays, 11(1990) the whole book especially 43-78, Elsevier, Amsterdam.
Shapiro, H., Practical Flow Cytometry, 3rdEdition, Wiley-Liss, New York, NY (1995) p. 277.
Haughland, R., Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes, Inc., Sixth Edition, Eugene, OR 97402 (1996).
Shapiro, H., “Practical Flow Cytometry”, 3rd edition, Wiley-Liss, New York, NY (1995) p. 277.

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