Fluorescent polarization immunoassay utilizing substituted triaz

Details Fluorescent polarization immunoassay utilizing substituted triaz Fluorescent polarization immunoassay utilizing substituted triaz

1983-10-31

1986-06-03

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 137, 536 14, 536 168, 536 174, 436536, C07H 15232, C07H 15234

Patent

active

045930899

ABSTRACT:
This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescent polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescent polarization techniques to provide a means for determining the amount of a specific ligand present in a sample.

REFERENCES:
patent: 4226978 (1980-10-01), Boguslaski et al.
patent: 4420568 (1983-12-01), Wang et al.
Sheldrick et al. "Reprint from Nature", vol. 271, No. 5642, pp. 223-225, Jan. 19, 1978.
Blakeslee et al., "Jour. of Immunological Methods", 13 (1976) pp. 305-320.
Watson et al., "Clinica Chimica Acta." vol. 73 (1976) pp. 51-5.
Spencer et al., "Clinical Chemistry", vol. 19, No. 8, 1973, pp. 838-844.
Blakeslee, "Jour. of Immunological Methods", 17 (1977) pp. 361-364.
McGregor et al., "Clinica Chimica Acta.", 83 (1978) pp. 161-166.
Kobayashi et al., "Clinica Chimica Acta", 92 (1979) pp. 241-247.

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