Fluorescent oligonucleotides and uses thereof

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S091100, C435S091200, C435S091210, C536S063000, C536S023100, C536S024300, C536S024310, C536S024320, C536S024330, C536S025300, C422S050000, C422S052000, C422S051000, C422S051000, C422S051000, C702S020000

Reexamination Certificate

active

06838244

ABSTRACT:
Oligonucleotide molecules labeled with a plurality of fluorophores of one or more types embedded in the backbone of said oligonucleotide, wherein at least one of said fluorophores is not located at either the 3′ or 5′ terminus of said oligonucleotide. The invention further provide methods for using the subject labeled oligonucleotides in detecting biological molecules, sequencing DNA molecules and particularly generating cDNA molecules for characterizing the differential expression of genes.

REFERENCES:
patent: 4458066 (1984-07-01), Caruthers et al.
patent: 5804386 (1998-09-01), Ju
patent: 5830658 (1998-11-01), Gryaznov
patent: 5853992 (1998-12-01), Glazer et al.
patent: 6060240 (2000-05-01), Kamb et al.
patent: 20020051981 (2002-05-01), Getts
1998 Qiagen catalog (pp. 7-8).*
Stears et al., “A novel, sensitive detection system for high-density microarrays using dendrimer technology”,Physical Genomics, 3:93-99 (2000).
Beaucage, S.L., et al., “Deoxynucleoside Phosphoramidities-A New Class of Key Intermediates for Deoxypolynucleotide Synthesis”,Tetrahedron Letters, 22:1859-1962, (1981).
DeRisi, J.L., et al., “Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale”,Science, 278:680-686, (1997).
Heller, R.A., et al., “Discovery and Analysis of Inflammatory Disease-Related Genes Using cDNA Microarrays”,Proc. Natl. Acad. Sci. USA, 94:2150-2155, (1997).
Liang, P., et al., “Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction”,Science, 257:967-971, (1992).
Matteucci, M.D., et al., “Synthesis of Deoxyoligonucleotides on a Polymer Support”J. Am. Chem. Soc., 103:3185-3191, (1981).
Prober, J.M., et al., “A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides”Science, 238:336-341, (1987).
Ruan, Y., et al., “Towards Arabidopsis Genome Analysis: Monitoring Expression Profiles of 1400 Genes Using cDNA Microarrays”,The Plant Journal, 15:821-833 (1998).
Schena, M., et al., “Quantative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray”,Science, 270:467-470, (1995).
Schena, M., et al., “Parallel Human Genome Analysis: Microarray-based Expression Monitoring of 1000 Genes”,Proc. Natl. Acad. Sci. USA, 93:10614-10619 (1996).
Smith, L.M., et al., “Fluorescence Detection in Automated DNA Sequence Analysis”,Nature, 321:674-679, (1986).
Wu, R. (ed.), “Methods in Enzymology” 155:51-334, (1987).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Fluorescent oligonucleotides and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescent oligonucleotides and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescent oligonucleotides and uses thereof will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3413007

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.