Fluorescent nucleotides and labeling methods using the same

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023100, C536S025300, C435S006120, C435S091100

Reexamination Certificate

active

07087744

ABSTRACT:
Fluorescent nucleotides useful for labeling nucleic acids which are represented by the formula: X—Y—Z wherein X represents a residue of natural or non-natural nucleotide and the like and binds to Y at a basic moiety of the residue; Y represents a divalent bridging group or a single bond; Z represents a monovalent group derived from a compound represented by the formula (I) wherein R1to R4represent an alkyl group; V1to V6represent a hydrogen atom or a substituent; L1to L7represent a methine group; W represents an oxygen atom, a sulfur atom, —C(R3)(R4)— or —N(R5)— wherein R3to R5represents an alkyl group; Q represents a nitrogen atom or —C(V7)— wherein V7represents a hydrogen atom or a monovalent substituent; M represents a counter ion; m represents a number required to neutralize the charge of the molecule; s, t and u represent 0 or 1 or the like, and binds to Y at a reactive group existing in R1or R2

REFERENCES:
patent: 5476928 (1995-12-01), Ward et al.
patent: 5569587 (1996-10-01), Waggoner
patent: 2002/0064794 (2002-05-01), Leung et al.
patent: 870753 (1961-06-01), None
patent: 2-191674 (1990-07-01), None
patent: 3-146565 (1991-06-01), None

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