Fluorescent nucleotide analogues

Details Fluorescent nucleotide analogues Fluorescent nucleotide analogues

2005-09-27

2008-11-04

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S007200, C536S004100, C536S026600

Reexamination Certificate

active

07445902

ABSTRACT:
The present invention provides fluorescent cyanine dye-based nucleotide analogues, in which the cyanine dye is coupled to the γ-phosphate group of a nucleoside triphosphate. Preferred embodiments of the invention are directed to fluorescent cyanine dye-based GTP analogues which may be employed in an homogeneous FRET-based assay to measure the binding of guanine nucleotides to GPCR polypeptides, or alternatively, to measure the effect of an exogenous ligand on GPCR protein binding.

REFERENCES:
patent: 5268486 (1993-12-01), Waggoner et al.
patent: 6323186 (2001-11-01), Klaubert et al.
patent: 6573374 (2003-06-01), Muehlegger et al.
patent: 2003/0186348 (2003-10-01), Thomas et al.
patent: 1042407 (2001-12-01), None
patent: WO02/26891 (2002-04-01), None
patent: WO02/086088 (2002-10-01), None
patent: WO2005/024380 (2005-03-01), None
patent: WO2006/035208 (2006-04-01), None
Anonymous. (2004). Jena Bioscience Product Catalog: XP-002369683. (Online), retrieved Feb. 24, 2006 from http://www.jenabioscience.com/index.php/8e86af4e8f099e545bc047e0ee40a6bc/1/browse/345>.
Arai, Y., Sugawa, M., Iwane, A. H. & Yanagida, T. (Aug. 2004). “Dynamic polymorphism of a cellular signal protein Ras studied by single molecule FRET: How does Ras interact with many effectors to regulate signaling pathways?” XP-002369620. Abstracts of Papers American Chemical Society, 228(2), U206. & Abstract. (2004). [228th Meeting of the Division of Chemical Toxicology of the American Chemical Society, held Aug. 22-26, 2004, Philadelphia, PA]. ISSN: 0065-7727.
Adhikari, A. & Sprang, S. R. (Dec. 19, 2003). “Thermodynamic Characterization of the Binding of Activator of G Protein Signaling 3 (AGS3) and Peptides Derived from AGS3 with Gαi1*”. The Journal of Biological Chemistry, 278(51), 51825-51832. & Chemical Abstract. (2005). ACS Online CAPLUS Abstract No. 140:195104. American Chemical Society on STN.
Cunliffe, J., Liu, Z., Pawliszyn, J. & Kennedy, R. (2004). “Use of a native ligand for the detection of G proteins by capillary isoelectric focusing with laser-induced fluorescence detection”. Electrophoresis, 25(14), 2319-2325. & Chemical Abstract. (2005). ACS Online CAPLUS Abstract No. 141:327911. American Chemical Society on STN.
Draganescu, A., Hodawadekar, S. C., Gee, K. R. & Brenner, C. (2000). “Fhit-nucleotide Specificity Probed with Novel Fluorescent and Fluorogenic Substrates*”. The Journal of Biological Chemistry, 275(7), 4555-4560. & Chemical Abstract. (2005). ACS Online CAPLUS Abstract No. 132:305036. American Chemical Society on STN.
Gellibolian, R. & Rouhani, R. (2005). Chemical Abstract. “Assaying transferase activity by using an artificial, multifunctional substrate comprising small-molecule component linked to biopolymer-substrate-mimetic component”. ACS Online CAPLUS Abstract Accession No. 2005:239208. American Chemical Society on STN.
Jameson, E. E., Cunliffe, J. M., Neubig, R. R., Sunahara, R. K. & Kennedy, R. T. (Aug. 15, 2003). “Detection of G Proteins by Affinity Probe Capillary Electrophoresis Using a Fluorescently Labeled GTP Analogue”. Analytical Chemistry, 75(16), 4297-4304. & Chemical Abstract. (2005). ACS Online CAPLUS Abstract No. 139-226677. American Chemical Society on STN.
Klaubert, D. H., Gee, K. R. & Brenner, C. M. (2005). Chemical Abstract. “Phosphate-bound polyazaindacene derivatives of nucleotides”. ACS Online CAPLUS Abstract No. 136:2485. American Chemical Society on STN.
McEwen, D. P., Gee, K. R., Kang, H. C. & Neubig, R. R. (2001). “Fluorescent BODIPY-GTP Analogs: Real-Time Measurement of Nucelotide Binding to G Proteins”. Analytical Biochemistry, 291(1), 109-117. & Chemical Abstract. (2005). ACS Online CAPLUS Abstract No. 135:58010. American Chemical Society on STN.
Muehlegger, K., Hoeltke, H. Birkner, C. & Eltz, H. V. (2005). Chemical Abstract. “Preparation of infrared dye-marked nucleotides for marking, detection, and sequencing of nucleic acids”. ACS Online CAPLUS Abstract No. 123:286528. American Chemical Society on STN.
Ramachandran, S. & Cerione, R. A. (2004). “Stabilization of an Intermediate Activation State for Transducin by a Fluorescent GTP Analogue”. Biochemistry, 43(27), 8778-8786. & Chemical Abstract. (2005). ACS Online CAPLUS Abstract No. 141:169674. American Chemical Society on STN.
Whelan, R., Sunahara, R. K., Neubig, R. R. & Kennedy, R. T. (2004). “Affinity Assays Using Fluorescence Anisotropy with Capillary Electrophoresis Separation”. Analytical Chemistry, 76(24), 7380-7386. & Chemical Abstract. (2005). ACS Online CAPLUS Abstract No. 142:109770. American Chemical Society on STN.
Williams, J. G. K. (2005). Chemical Abstract. “DNA polymerase mutants with increase activity for charge-switch nucloetides”. ACS Online CAPLUS Abstract No. 137:347515. American Chemical Society on STN.

Affiliated with

Also associated with

Rating

Fluorescent nucleotide analogues does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescent nucleotide analogues, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescent nucleotide analogues will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4026137