Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2004-10-28
2008-09-30
Horlick, Kenneth R. (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S024300, C536S026700, C536S026800
Reexamination Certificate
active
07429651
ABSTRACT:
Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators.
REFERENCES:
patent: 4795699 (1989-01-01), Tabor et al.
patent: 5047519 (1991-09-01), Hobbs, Jr. et al.
patent: 5558991 (1996-09-01), Trainor
patent: 5986086 (1999-11-01), Brush et al.
patent: 6248531 (2001-06-01), Houthoff et al.
patent: 6340747 (2002-01-01), Bazin et al.
patent: 00731178 (1996-09-01), None
patent: WO9319078 (1993-09-01), None
patent: WO9611937 (1996-04-01), None
patent: WO9858942 (1998-12-01), None
patent: WO9918114 (1999-04-01), None
patent: WO9937810 (1999-07-01), None
patent: WO9940223 (1999-08-01), None
patent: WO0119841 (2001-03-01), None
Confalone, J. Heterocyclic Chem., 1990, 27, 31-46.
Finn et al., Nucleic Acids Res., 2002, 30(13), 2877-2885.
Rosenblum B.B. et al., New dye-labeled terminators for improved DNA sequencing patterns, Nucleic Acids Research, 1997, 25 (22), 4500-4504.
Schoetzau et al., Perkin Trans. 1, J. Chem. Soc., 2000, 1411-1415.
Stolze et al., Helvetica Chimica Acta, 1999, 82, 1311-1323.
Yamaguchi et al., Nucleosides and Nucleotides, Oct. 19, 2001, 15, 607-618.
Khan Shaheer H.
Menchen Steven M.
Rosenblum Barnett B.
Taing Meng
Applera Corporation
Horlick Kenneth R.
Liptak Vincent P.
Recipon Shirley A.
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