Fluorescent material labeled-probe and method for detecting hybr

Details Fluorescent material labeled-probe and method for detecting hybr Fluorescent material labeled-probe and method for detecting hybr

1998-03-12

2000-06-20

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, C12Q 168

Patent

active

060776706

ABSTRACT:
A method is disclosed which is capable of increasing a S/N ratio without subjecting a sample to any treatment to thereby detect hybridization with high sensitivity. A fragmentarily divided probe is used which is prepared by dividing a single probe 12 into a plurality of fragmentary probes 12a, 12b, and labeling the fragmentary probes with different fluorescent materials 14a, 14b, respectively. When the probe is hybridized with a sample 11, the fragmentary probes 12a,12b reconstitute the single probe. An intermittent light beam is used as excitation light 19. The fluorescent material 14a is excited therewith, and fluorescence 18 emitted from the fluorescent material 14b through multistep excitation is detected while irradiation with the excitation light is intermitted.

REFERENCES:
patent: 4996143 (1991-02-01), Heller et al.
patent: 5688648 (1997-11-01), Mathies et al.
patent: 5849489 (1998-12-01), Heller
Matthews et al., Analytical Biochemistry, vol. 169, pp. 1-25, 1988.

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