Fluorescent lipid polymer-macromolecular ligand compositions as

Details Fluorescent lipid polymer-macromolecular ligand compositions as Fluorescent lipid polymer-macromolecular ligand compositions as

1993-07-09

1995-05-16

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 791, 435 792, 435 794, 435 18, 435 21, 435968, 436518, 436805, G01N 3353

Patent

active

054159997

ABSTRACT:
Methods and compositions are provided for the detection of analytes. The method employs a fluorescence production layer which comprises a fluorescent polymerized polyunsaturated lipid layer in association with a ligand which is a member of a specific binding pair, where the ligand is competitive with the analyte for the complementary binding member or is a complementary binding member. By providing for a fluorescence modulation reagent which binds to the fluorescence production layer in proportion to the amount of analyte in the sample, by measuring the resulting fluorescence after carrying out the assay methodology, the amount of analyte can be determined quantitatively.

REFERENCES:
patent: 4489133 (1984-12-01), Kornberg
patent: 5268305 (1993-12-01), Ribi et al.
T. E. Wilson et al., Langmuir, vol. 8, No. 10, pp. 2361-2364, 1992.
Bader et al., "Polymeric Monolayers and Liposomes as Models for Biomembranes", Advances in Polymer Science, 64:1-62 (1985).
Hashida et al., "More Useful Maleimide Compounds for the Conjugation of Fab' to Horseradish Peroxidase through Thiol Groups in the Hinge", J. of Applied Biochemistry, 6:56-63 (1984).
Laguzza et al., "New Antitumor Monoclonal Antibody-Vinca Conjugates LY203725 and Related Compounds: Design, Preparation, and Representative in Vivo Activity", J. Med. Chem., 32:548-555 (1989).
Lochner et al., "Photoconduction in Polydiacetylene Multilayer Structures and Sincle Crystals", Phys. Stat. Solidi, 88:653-661 (1978).
Miller et al., "Antibody Properties for Chemically Reversible Biosensor Applications", Anal. Chim. Acta., 227:135:143 (1989).
O'Shannessey et al., "Specific Conjugation Reactions of the Oligosaccharide Moieties of Immunoglobulins", J. Appl. Bioch., 7:347-355 (1985).
Owen et al., "Non-Electrode Biosensors in Clinical Biochemistry", Ann. Clin. Biochem., 22:559-564 (1985).
Sugi., "Langmuir-Blodgett Films-a Course Towards Molecular Electronics: a Review", J. Molecular Electronics, 1:3-17 (1985).
Thompson et al., "Biosensors and Bioprobes", Trends in Anal. Chem., 3(7):173-178 (1984).
Aime et al., "Study of the Pressure Effect on Polydiacetylene in Good Solvent," Synthetic Metals (1991)41-43 203-207.
Batchelder et al., "A Compact Raman Microprobe/Microscope: Analysis of Polydiacetylene Langmuir and Langmuir Blodgett Films," Makromol. Chem., Macromol. Symp. (1991) 46: 171-179.
Chu & Xu, "Chromatic Transition of Polydiacetylene in Solution," Acc. Chem. Res. (1991) 24:384-389.
Takeda et al., "Molecular Design for Reversible Phase Transition Systems Based on Polydiacetylenes," Synthetic Metals (1991) 41-43: 231.

Affiliated with

Also associated with

Rating

Fluorescent lipid polymer-macromolecular ligand compositions as does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescent lipid polymer-macromolecular ligand compositions as , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescent lipid polymer-macromolecular ligand compositions as will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-637175