Fluorescent lipid polymer-macromolecular ligand compositions

Details Fluorescent lipid polymer-macromolecular ligand compositions Fluorescent lipid polymer-macromolecular ligand compositions

1995-03-16

1997-04-08

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

422 57, 422 60, 435 79, 435 791, 435 792, 435 794, 435968, 436805, G01N 3353, G01N 33543

Patent

active

056187351

ABSTRACT:
Methods and compositions are provided for the detection of analytes. The method employs a fluorescence production layer which comprises a fluorescent polymerized polyunsaturated lipid layer in association with a ligand which is a member of a specific binding pair, where the ligand is competitive with the analyte for the complementary binding member or is a complementary binding member. By providing for a fluorescence modulation reagent which binds to the fluorescence production layer in proportion to the amount of analyte in the sample, by measuring the resulting fluorescence after carrying out the assay methodology, the amount of analyte can be determined quantitatively.

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