Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1994-02-01
1997-08-05
Jones, W. Gary
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 435810, 436501, 436 63, 536 221, 536 231, 935 77, 935 78, C07H 2100, C12Q 168
Patent
active
056544197
ABSTRACT:
Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.
REFERENCES:
patent: 4996143 (1991-02-01), Heller et al.
patent: 5188934 (1993-02-01), Menchen et al.
patent: 5326692 (1994-07-01), Brinkley et al.
Geysen et al. (1988) Journal of Molecular Recognition, vol. 1, No. 1, pp. 32-41.
Ansorge et al., "Automated DNA Sequencing: Ultrasensitive Detection of Fluorescent Bands During Electrophoresis", Nucleic Acids Research 15(11):4593-4602 (1987).
Cardullo et al., "Detection of Nucleic Acid Hybridization by Nonradiative Fluorescence Resonance Energy Transfer", Proc. Natl. Acad. Sci. USA 85:8790-8794 (1988).
Clegg et al., "Fluorescence Resonance Energy Transfer Analysis of the Structure of the Four-Way DNA Junction", Biochemistry 31(20):4846-4856 (1992).
Huang et al., "DNA Sequencing Using Capillary Array Electrophoresis", Anal. Chem. 64(18):2149-2154 (1992).
Maliwal et al., "End-to-end Diffusion Coefficients and Distance Distributions from Fluorescence Energy Transfer Measurements: Enhanced Resolution by Using Multiple Acceptors with Different Forster Distances", Biophysical Chemistry 46:273-281 (1993).
Mathies and Huang "Capillary Array Electrophoresis: An Approach to High-speed, High-throughput DNA Sequencing", Nature 359:167-169 (1992).
Middendorf et al., "Continuous, On-line DNA Sequencing Using a Versatile Infrared Laser Scanner/Electrophoresis Apparatus", Electrophoresis 13:487-494 (1992).
Montenay-Garestier et al., "Design of Bifunctional Nucleic Acid Ligands", Molecular Basis of Specifity in Nucleic Acid-Drug Interactions, pp. 275-290 (1990), B. Pullman and J. Jortner (eds).
Prober et al., "A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides", Science 238:336-341 (1987).
Ruth et al., "Linker Arm Nucleotide Analogs Useful in Oligonucleotide Synthesis", DNA 4:93 (1985) (Abstract).
Sanger et al., "DNA Sequencing with Chain-terminating Inhibitors", Proc. Natl. Acad. Sci. USA 74(12):5463-5467 (1977).
Smith et al., "The Synthesis of Oligonucleotides Containing an Aliphatic Amino Group at the 5' Terminus: Synthesis of Fluorescent DNA Primers for Use in DNA Sequence Analysis", Nucleic Acids Research 13(7):2399-2412 (1985).
Smith et al., "Fluorescence Detection in Automated DNA Sequence Analysis", Nature 321:674-679 (1986).
Stryer, "Fluorescence Energy Transfer as a Spectroscopic Ruler", Ann. Rev. Biochemistry 47:819-846 (1978).
Stryer and Haugland, "Energy Transfer: A Spectroscopic Ruler", Proc. Natl. Acad. Sci. USA 58:719-726 (1967).
Theisen et al., "Fluorescent Dye Phosphoramidite Labeling of Oligonucleotides", Tetrahedron Letters 33(35):5033-5036 (1992).
Glazer Alexander
Ju Jingyue
Mathies Richard A.
Jones W. Gary
Marschel Ardin H.
Rowland Bertram I.
The Regents of the University of California
LandOfFree
Fluorescent labels and their use in separations does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Fluorescent labels and their use in separations, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescent labels and their use in separations will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1075930