Fluorescent labelled carbohydrates and their analysis

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

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204451, 204461, 204603, 204601, 204612, G01N 2726

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active

055693660

ABSTRACT:
Processes for the electrophoretic analysis of monosaccharides and/or polysaccharides are disclosed. The processes generally first include providing a composition having derivatized mono and/or polysaccharide components which are monosaccharide and/or polysaccharide labelled with a fluorescing charged compound. Then introducing the composition into an electrophoretic medium and applying an electric field across the electrophoretic medium causes the derivatized monosaccharide and/or polysaccharide components to differentially migrate within the electrophoretic medium. Then detecting the derivatized monosaccharide and/or polysaccharide components is accomplished by laser exciting the derivatized monosaccharide and/or polysaccharide components and monitoring fluorescent emission of the derivatized monosaccharide and/or polysaccharide components. The processes provide extremely low detection limits of the labelled components.

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Intro. to Modern Biochemistry, Academic Press, 1970, 3rd ed., Peter Karlson, p. 7.
Survey of Organic Syntheses, John Wiley, Calvin Buehler, 1973, p. 427.

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