Fluorescent labeling complexes with large stokes shift formed by

Details Fluorescent labeling complexes with large stokes shift formed by Fluorescent labeling complexes with large stokes shift formed by

1995-06-07

1999-12-28

Higel, Floyd D.

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

544212, 544328, 544455, 546272, 546273, 436 6, 535 6, 536800, C09B 4704, C09B 2300, C09B 2301, C09B 2304, G01N 33546

Patent

active

060083735

ABSTRACT:
The present invention provides low molecular weight fluorescent labeling complexes with large wavelength shifts between absorption of one dye in the complex and emission from another dye in the complex. These complexes can be used, for example, for multiparameter fluorescence cell analysis using a single excitation wavelength. The low molecular weight of the complex permits materials labeled with the complex to penetrate cell structures for use as probes. The labeling complexes are synthesized by covalently attaching through linkers at least one cyanine fluorochrome to another low molecular weight fluorochrome to form energy donor-acceptor complexes. Resonance energy transfer from an excited donor to fluorescent acceptor provides wavelength shifts up to 300 nm. The fluorescent labeling complexes preferably contain reactive groups for the labeling of functional groups on target compounds, such as derivatized oxy and deoxy polynucleic acids, antibodies, enzymes, proteins and other materials. The complexes may also contain functional groups permitting covalent reaction with materials containing reactive groups.

REFERENCES:
patent: 4745285 (1988-05-01), Recktenwald et al.
patent: 4774339 (1988-09-01), Haughland et al.
patent: 4876190 (1989-10-01), Recutenwald
patent: 4900686 (1990-02-01), Arnost et al.
patent: 5248782 (1993-09-01), Haughland et al.
patent: 5268486 (1993-12-01), Waggoner et al.
patent: 5274113 (1993-12-01), Kang et al.
patent: 5312921 (1994-05-01), Glazer et al.
patent: 5332662 (1994-07-01), Ullman
patent: 5378634 (1995-01-01), Nobuyuki et al.
patent: 5453505 (1995-09-01), Lee et al.
Carmel et al, FEBS Letters, vol. 30, No. 1, p. 11(1973).
DeBiasio et al, The Journal of Cell Biology, vol. 105, pp. 1613 to 1621 (1987).
Gale et al, Aust. J. Chem., vol. 30, pp. 689 to 694 (1977).
Hamer, The Cyanine Dyes and Related Compounds, pp. 32 to 85(1964).
Ol et al, Rapid Communications, pp. 981 to 986(1982).
Linda G. Lee, et al., DNA Sequencing with Dye-Labeled Terminators and T7 DNA Polymerase: Effect of Dyes and dnTPs on Incorporation of Dye-Terminators and Probability Analysis of Termination Fragments, Nucleic Acids Research, vol. 20, No. 10, pp. 2471-2483, Apr. 3, 1992.
A. I. Kiprianov and G. G. Dyadyusha Absorption Spectra of Organic Dyes Containing Two Conjugated Chromophores in the Molecule, Ukrainskii Khimicheskii Zhurnal, vol. 35, No. 6, pp. 608-615, 1969.
Lubert Stryer and Richard P. Haugland Energy Transfer: A spectroscopic Ruler, Proc. N.A.S., vol. 58, pp. 719-726, 1967.
Ratnakar B. Mujumdar et al. Cyanine Dye Labeling Reagents: Sulfoindocyanine Succinimidyl Esters, Bioconjugate Chem., vol. 4, No. 2, pp. 105-111, 1993.
Ratnakar B. Mujumdar et al. Cyanine Dye Labeling Reagents Containing Isothiocyanate Groups.sup.1, Cytometry, vol. 10, pp. 11-19, 1989.
Lauren A. Ernst et al. Cyanine Dye Labeling Reagents for Sulfhydryl Groups.sup.1, Cytometry, vol. 10, pp. 3-10, 1989.
Philip L. Southwick et al. Cyanine Dye Labeling Reagents--Carboxymethylindocyanine Succinimidyl Esters.sup.1, Cytometry, vol. 11, pp. 418-430, 1990.
Alan S. Waggoner et al. A New Fluorescent Antibody Label for Three-Color Flow Cytometry with a Single Laser.sup.a, Reprinted from Clinical Flow Cytometry, vol. 677 of the Annals of the New York Academy of Sciences, pp. 185-193, Mar. 20, 1993.
Jonathan S. Lindsey et al. Visible Light-Harvesting in Covalently-Linked Porphyrin-Cyanine Dyes, Tetrahedron, vol. 45, No. 15, pp. 4845=4866, 1989.

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