Fluorescent indicator proteins

Details Fluorescent indicator proteins Fluorescent indicator proteins

2006-07-25

2008-10-07

Gupta, Anish (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C536S023700, C435S014000, C435S069100, C435S069700

Reexamination Certificate

active

07432353

ABSTRACT:
A protein biosensor having increased signal intensity to sugar concentration and the use thereof. A fluorescent indicator protein is described, in which fluorescent proteins of different emission ranges are fused to both ends of a binding protein undergoing a conformational change due to binding of sugars, such that a change in the concentration of various sugars involved in intracellular metabolism, e.g., maltose, can be detected by measuring a change in emission intensities, caused by FRET (fluorescence resonance energy transfer). A method for detecting a change in the concentration of sugars using such fluorescent indicator protein is also described. The fluorescent indicator protein has excellent signal intensity enabling precision measurement of intracellular concentration of various sugars. In one implementation, high signal intensity fluorescent indicator proteins can be prepared from proteins otherwise used as biosensors, to enable them to be more widely useful.

REFERENCES:
patent: 6911199 (2005-06-01), Vigne et al.
patent: WO-98/40477 (1998-09-01), None
patent: WO-03/025220 (2003-03-01), None
patent: WO-2005/036178 (2005-04-01), None
GenBank Accession No. NP—626482.
GenBank Accession No. CAB65651.
Chauduri, Bernali Neel, et al., Structure of -allose binding protein fromEscherichia colibound to -allose at 1.8 Å resolution, J. Mol. Biol., Mar. 12, 1999, pp. 1519-1531, vol. 286, No. 5.
De Lorimier, Robert M., et al., Construction of a fluorescent biosensor family , Protein Science, Nov. 2002, pp. 2655-2675, vol. 11, No. 11.
Deuschle, Karen, et al., Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering , Protein Science, Sep. 2005, pp. 2304-2314, vol. 14, No. 9.
Dong, Min, et al., Using fluorescent sensors to detect botulinum neurotoxin activity in vitro and in living cells , Proc. Natl. Acad. Sci. USA, Oct. 12, 2004, pp. 14701-14706, vol. 101, No. 41.
Fehr, Marcus, et al., Visualization of maltose uptake in living yeast cells by fluorescent nanosensors , Proc. Natl. Acad. Sci. USA, Jul. 23, 2002, pp. 9846-9851, vol. 99, No. 15.
Fehr, Marcus, et al., In Vivo Imaging of the Dynamics of Glucose Uptake in the Cytosol of COS-7 Cells by Fluorescent Nanosensors, J. Biol. Chem., May 23, 2003, pp. 19127-19133, vol. 278, No. 21.
Fehr, Marcus, et al., Minimally invasive dynamic imaging of ions and metabolites in living cells, Curr. Opin. Plant Biol., Jun. 2004, pp. 345-351, vol. 7, No. 3.
Fukada, Harumi, et al., Thermodynamics of the binding of L-arabinose and of D-galactose to the L-arabinose-binding protein ofEscherichia coli, J. Biol. Chem., Nov. 10, 1983, pp. 13193-13198, vol. 258, No. 21.
Guntas, Gurkan, et al., Creation of an Allosteric Enzyme by Domain Insertion , J. Mol. Biol., Feb. 6, 2004, pp. 263-273, vol. 336, No. 1.
Guntas, Gurkan, et al., Directed evolution of protein switches and their application to the creation of ligand-binding proteins, Proc. Natl. Acad. Sci. USA, Aug. 9, 2005, pp. 11224-11229, vol. 102, No. 32.
Kim, Changhoon, et al., The D-allose operon ofEscherichia coliK-12, J. Bacteriol., Dec. 1997, pp. 7631-7637, vol. 179, No. 24.
Lager, Ida, et al., Development of a fluorescent nanosensor for ribose , FEBS Letters, Oct. 9, 2003, pp. 85-89, vol. 553, No. 1-2.
Looger, Loren L., et al., Computational design of receptor and sensor proteins with novel functions, Nature, May 8, 2003, pp. 185-190, vol. 423, No. 6936.
Magnusson, Ulrika, et al., Hinge-bending Motion of D-Allose-binding Protein fromEscherichia coli, J. Biol. Chem., Apr. 19, 2002, pp. 14077-14084, vol. 277, No. 16.
Marvin, J.S., et al., The rational design of allosteric interactions in a monomeric protein and its applications to the construction of . . . , Proc. Natl. Acad. Sci. USA, Apr. 1997, pp. 4366-4371, vol. 94.
Miyawaki, Atsushi, et al., Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin, Nature, Aug. 28, 1997, pp. 882-887, vol. 388.
Mochizuki, Naoki, et al., Spatio-temporal images of growth-factor-induced activation of Ras and Rap1, Nature, Jun. 28, 2001, pp. 1065-1068, vol. 411, No. 6841.
Nagai, Takeharu, et al., Circularly permuted green fluorescent proteins engineered to sense Ca2+, Proc. Natl. Acad. Sci. USA, Mar. 13, 2001, pp. 3197-3202, vol. 98, No. 6.
Nagai, Takeharu, et al., Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins , Proc. Natl. Acad. Sci. USA, Jul. 20, 2004, pp. 10554-10559, vol. 101, No. 29.
Okumoto, Sakiko, et al., Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors , Proc. Natl. Acad. Sci. USA, Jun. 14, 2005, pp. 8740-8745, vol. 102, No. 24.
Patterson, George H., et al., Förster Distances between Green Fluorescent Protein Pairs , Anal. Biochem., Sep. 10, 2000, pp. 438-440, vol. 284, No. 2.
Patterson, George, et al., Fluorescent protein spectra , J. Cell Sci., Mar. 1, 2001, pp. 837-838, vol. 114, No. 5.
Sohn, J.-H., et al., Process Development for the Production of Recombinant Hirudin inSaccharomyces cerevisiae: from Upstream to Downstream, Process Biochemistry, 1995, pp. 653-660, vol. 30, No. 7.
Spurlino, J.C., et al., The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active . . . , J. Biol. Chem., Mar. 15, 1991, pp. 5202-5219, vol. 266, No. 8.
Tanimura, Akihiko, et al., Fluorescent Biosensor for Quantitative Real-time Measurements of Inositol 1,4,5-Trisphosphate in Single Living Cells, J. Biol. Chem., Sep. 10, 2004, pp. 38095-38098, vol. 279, No. 37.

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