Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Patent
1987-10-30
1991-09-17
Raymond, Richard L.
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
546255, 546256, 546261, 546265, 546269, 546272, 546274, 546285, 546296, 546300, 546301, 546302, 548517, 548518, 548525, 548527, 548528, 548541, 549 2, 549 21, 549 26, 549 27, 549 28, 549 59, 549 60, 549 65, 549223, 549225, 549227, 549415, 549417, 549472, 549476, 260351, C07D21168, C07C21908, C07C31182, C07C10142, C07C20700
Patent
active
050496735
ABSTRACT:
The present invention discloses a new class of calcium specific fluorescent indicator dyes having visible excitation and emission wavelengths. The new fluorescent indicator dyes combine at least one tricyclic chromophore with a tetracarboxylate parent CA.sup.2+ chelating compound having the octacoordinate pattern of liganding groups characteristic of BAPTA to give a rhodamine-like or fluorescein-like fluorophore. Binding of calcium.sup.2+ increases the fluorescence of the new compounds by up to 40-fold. The calcium.sup.2+ dissociation constants are in the range 0.37-2.3 microM, so that the new indicators give better resolution of high [CA.sup.2+ ] levels than were previously obtainable with predecessor compounds such as quin-2 or fluo-2. The visible excitation wavelengths of the new compounds are more convenient for fluorescent microscopy and flow cytometry than the UV required by previous indicators.
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Minta Akwasi
Tsien Roger Y.
Covington Raymond
Raymond Richard L.
The Regents of the University of California
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