Fluorescent in situ RT-PCR

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091210, C536S024330

Reexamination Certificate

active

10149461

ABSTRACT:
The present invention describes an in situ reverse transcriptase PCR method in which the background fluorescence is greatly reduced as compared to traditional in situ PCR.

REFERENCES:
patent: 4999293 (1991-03-01), Barsomian et al.
patent: 5538871 (1996-07-01), Nuovo et al.
patent: 5804383 (1998-09-01), Gruenert et al.
patent: 5853697 (1998-12-01), Strober et al.
patent: 5962477 (1999-10-01), Mak
patent: 5989873 (1999-11-01), Vinayagamoorthy et al.
patent: 6218523 (2001-04-01), French et al.
patent: 6270966 (2001-08-01), Weinstein et al.
patent: 6451530 (2002-09-01), Hawkins
Jena et al in “PCR Based RFLP (PBR) as Genetic Marker for Hybrid Rice Improvement” Plant and Animal Genome V Conference, Jan. 12-16, 1997.
Stratagene Catalog. cDNA synthesis kit. Stratagene Catalog, p. 123, 1995.
Bagasra et al. Application of in situ PCR techniques to human tissues. PCR3—PCR in situ hybridization a practical approach. pp. 117-138, 1998.
Vazquez et al. Enhancement of PCRs by partial restriction digestion of genomic templates. Biotechniques, Vo. 26 (1), pp. 9 95, 1999.
Salian et al. Polymerase chain reaction to detect mycobacterium tuberculosis in histological specimens. Am J Respir Crit Care Med., vol. 158, pp. 1150-1155, 1998.
Stratagene Catalog. Methylases. Stratagene Catalog, p. 305, 1995.
Neumaier M, et al. Fundamentals of quality assessment of molecular amplification methods in clinical diagnostics. Clin Chem., vol. 44 (10, p. 12-26, 1998.
Garner SC, et al. Quantitative analysis of the calcium-sensing receptor messenger RNA in parathyroid adenomas. Surgery, vol. 122 (6), p. 1166-1175, 1997.
Bagastra et al., “Detection of Human Immunodeficiency Virus Type I Provirus in Mononuclear Cells byin situPolymerase Chain Reaction,”New Engl. J. Med.,326:1385-91 (1992).
Boshoff et al., “Kaposi's Sarcoma-Associated Herpesvirus Infects Endothelial and Spindle Cells,”Nature Medicine, 1:1274-1278 (1995).
Chen et al., “The Use of Granzyme A as a Marker of Heart Transplant Rejection in Cyclosporine or Anti-CD4 Monoclonal Antibody-Treated Rats,”Transplantation, 55:146-153 (1993).
Chiu et al., “Intracellular Amplification of Proviral DNA in Tissue Sections Using the Polymerase Chain Reaction,”Journal of Histochemistry&Cytochemistry, 40:333-341 (1992).
Embleton et al., “In-Cell PCR from mRNA: Amplifying and Linking the Rearranged Immunoglobulin Heavy and Light Chain V-Genes with Single Cells,”Nucleic Acids Research, 20:3831-3834 (1992).
Ertsey and Scavo, “Coverslip Mounted-Immersion Cycledin situRT-PCR for the Localization of mRNA in Tissue Sections,”Biotechniques, 24:92, 94, 96, 98-100 (1998).
Fleming, “Analysis of Viral Pathogenesis by in situ Hybridization,”Journal of Pathology, 166: 95-96 (1992).
Haase et al., “Amplification and Detection of Lentiviral DNA Inside Cells,”Proc. Nat'l Acad. Sci. USA, 87:4971-4975 (1990).
Heniford et al., “Variation in Cellular EGF Receptor mRNA Expression Demonstrated byin situReverse Transcriptase Polymerase Chain Reaction,”Nucleic Acids Research, 21:3159-3166 (1993).
Komminoth et al., “Evaluation of Method for Hepatitis C Virus Detection in Archival Liver Biopsies: Comparison of Histology, Immunohistochemistry, In-situ Hybridization, Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and In-situ RT-PCR,”Pathology, Research&Practice, 190:1017-1025 (1994).
Kuo et al., “Identification and Characterization of an RNA Specific Primer for Human Tumor Necrosis Factor Receptor-1 (TNFR-1),”Biochem.&Mol. Biol. Intern'l,40:119-125 (1996).
Lee et al., “Detection of Soluble Fas mRNA Using In-situ Revers Transcription-Polymerase Chain Reaction,”Laboratory Investigation, 78:453-459 (1998).
Long et al., “Comparison of Indirect and Direct In-situ Polymerase Chain Reaction in Cell Preparations and Tissue Sections: Detection of Viral DNA, Gene Rearrangements and Chromosal Translocations,”Histochemistry, 99:151-162 (1993).
Martinez et al., “Non-Radioactive Localization of Nucleic Acids by Direct In situ PCR and In-situ RT-PCR in Paraffin-Embedded Sections,”J. Hist.&Cytochem.,43:739-747 (1995).
Mee et al., “Quantification of Vitamin D Receptor mRNA in Tissue Sections Demonstrates the Relative Limitations of In situ-Reverse Transcriptase-Polymerase Chain Reaction,”J. Path.,182:22-28 (1997).
Negro et al., “Detection of Intrahepatic Replication of Hepatitis C Virus RNA by in situ Hybridization and Comparison with Histopathology,”Proc. Nat'l Acad. Sci. USA, 89:2247-2251 (1992).
Nuovo et al., “An Improved Technique for the in situ Detection of DNA after Polymerase Chain Reaction Amplification,”A.J. Path.,139:1239-1244 (1991).
Nuovo et al., “In situ Localization of PCR-Amplified Human and Viral cDNAs,”Genome Research, 2:117-23 (1992).
Nuovo, “PCR In situ Hybridization,”Methods in Molecular Biology, 33:223-241 (1994).
Nuovo, “The Nonspecific Pathways of PCR,” inPCR in situ Hybridization: Protocols and Applications, 2nded., Lippincott-Raven, Philadelphia, pp. 54-99, 1996.
O'Leary et al., “In situ PCR: Pathologist's Dream or Nightmare?,”Journal of Pathology, 178:11-20 (1996).
Patterson et al., “Detection of HIV-1 DNA and Messenger RNA in Individual Cells by PCR-Drivenin SituHybridization and Flow Cytometry,”Science, 260:976-979 (1993).
Patel et al., “Detection of Epidermal Growth Factor Receptor mRNA in Tissue Sections from Biopsy Specimens Usingin SituPolymerase Chain Reaction,”American Journal of Pathology, 144:7-14 (1994).
Peters et al., “Detection of Rare RNA Sequences by Single-Enzymein SituReverse Transcription Polymerase Chain Reaction: High Resolution Analyses of Interleukin-6 mRNA in Paraffin Sections of Lymph Nodes,”American Journal of Pathology, 150:469-476 (1997).
Singer et al., “Detection of HIV-1-Infected Cells from Patients Using Nonisotopicin SituHybridization,”Blood, 74: 2295-2301 (1989).
Staecker et al., “A Procedure for RT-PCR Amplification of mRNAs in Histological Specimens,”Biotechniques, 16:76-80 91994).
Tecott et al., “In SituTranscription: Specific Synthesis of Complementary DNA in Fixed Tissue Sections,”Science, 240:1661-1664 (1988).
Tolker-Nielsen et al., “Non-Genetic Population Heterogeneity Studies byin SituPolymerase Chain Reaction,”Molecular Microbiology, 27:1099-1105 (1998).

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