Fluorescent immunoassay employing a phycobiliprotein labeled lig

Details Fluorescent immunoassay employing a phycobiliprotein labeled lig Fluorescent immunoassay employing a phycobiliprotein labeled lig

1982-12-30

1985-05-28

Marantz, Sidney

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436546, 436536, 436519, 436800, 424 71, 260112R, 260112B, G01N 3354, G01N 3100

Patent

active

045201102

ABSTRACT:
Sensitive detection techniques and compositions for such techniques employing fluorescent proteins having bilin prosthetic groups as labels i.e. phycobiliprotein. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjuction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.

REFERENCES:
patent: 4193983 (1980-03-01), Ullman
patent: 4302536 (1981-11-01), Longenecker
Hales et al., "Labeled Antibodies and Their Use in the Immunoradiometric Assay", Immunochemical Techniques, vol. 7, Methods in Enzymology, 1980, pp. 334-355.
Kronick et al., "Immunoassay Techniques with Fluorescent Phycobiliprotein Conjugates", Clinical Chem., vol. 29, #9, 1983, pp. 1582-1586.
O'hEocha, Biochemistry, vol. 2, 1963, pp. 375-382.
Stedman's Medical Dictionary, Williams & Wilkins, Balt. 22nd Ed., 1972, pp. 154, 155, 318, 766, 1004

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