Fluorescent haloalkyl derivatives of reporter molecules well ret

Details Fluorescent haloalkyl derivatives of reporter molecules well ret Fluorescent haloalkyl derivatives of reporter molecules well ret

1993-03-05

1994-11-08

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 19, 435 20, 435 21, 435 22, 435 23, 435 24, 435 25, 435 26, 435 14, 435 29, 436 63, 436 66, 530300, 530802, 536 41, 536 181, C12Q 100, C12Q 134, G01N 3340, A61K 3702, C07H 1524

Patent

active

053626281

ABSTRACT:
The subject invention provides a method for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process involving conjugation of haloalkyl-substituted derivatives of a reporter molecule with intracellular cysteine-containing peptides while unblocking the reporter molecule. The method for analyzing metabolic activity of cells involves the use of a substrate having the form

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