Fluorescent gram stain

Details Fluorescent gram stain Fluorescent gram stain

1984-10-01

1987-01-27

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 36, 435 38, C12Q 104, C12Q 114, C12Q 110

Patent

active

046394214

ABSTRACT:
A method to determine the Gram sign of microorganisms includes staining the microorganisms with a plurality of fluorescent dyes in the presence of a staining buffer, applying excitation energy to the stained microorganisms, observing the color of the fluorescence emission of the stained microorganisms, and assigning the positive Gram sign to microorganisms which fluoresce substantially green and the negative Gram sign to microorganisms which fluoresce substantially orange.

REFERENCES:
patent: 4094745 (1978-06-01), Scholefield
patent: 4126516 (1978-10-01), Messing et al.
patent: 4225669 (1980-09-01), Melnick et al.
patent: 4508821 (1985-04-01), Mansour et al.
Matsuyama, Federation of European Microbiological Societies Microbiology Letters, vol. 21, 1984, pp. 153-157.
Boge et al. Journal & General Microbiology (1983), vol. 129, pp. 973-980.
Stohr et al., Histochemistry, vol. 51, 1977, pp. 305-313.
Govoronov et al., Mikrobiologiya 1982, vol. 51, No. 5 pp. 731-734._

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