Fluorescent fluid determination method and apparatus

Chemistry: analytical and immunological testing – Including titration or ph determination

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422 68, 436133, 436172, 436178, G01N 2164, G01N 2180

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active

045489076

ABSTRACT:
A fluorescence-based optical sensor includes a membrane immobilized fluorophor secured to one end of a bifurcated fiber optic channel for exposure to the sample to be analyzed. The fiber optic channel also has an input end coupled to a radiation source arranged to supply radiation at two different wavelengths and an output end coupled to a bandwidth limited photosensor. The radiation source alternately excites the fluorophor at a first wavelength that excites an acid form of the fluorophor and at a second wavelength that excites a base form of the fluorophor, and a ratio of the resulting fluorescence intensities is taken as a measure of a characteristic of the sample being analyzed.

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