Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Signal modification or steric inhibition
Patent
1994-01-19
1997-05-20
Green, Lora M.
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
Signal modification or steric inhibition
436536, 436518, 436546, 436172, 436800, 422 8207, 422 8208, 422 8209, G01N 33533
Patent
active
056311699
ABSTRACT:
A fluorometric luminescence immunoassay method includes forming a sample by exposing a first immune reaction reactant to a second immune reaction reactant capable of reacting with the first reactant, one of the first and second immune reaction reactants being labelled with a photoluminescent energy transfer donor and the other being labelled with a photoluminescent energy transfer acceptor complementary to the photoluminescent donor. At least the photoluminescent donor has the property of photoluminescence, and the photoluminescent donor and acceptor are chosen so that when the first immune reaction reactant reacts with the second immune reaction reactant, the donor and the acceptor are capable of interacting to produce a detectable luminescence lifetime change. The sample is excited with radiation, and the resulting emission is detected. The apparent luminescent lifetime is then calculated to determine the presence of a reaction product of the first and second immune reaction reactants.
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Lakowicz Joseph R.
Maliwal Badri P.
Ozinskas Alvydas
Thompson Richard
Green Lora M.
Lakowicz Joseph R.
Thompson Richard
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