Fluorescent dye

Details Fluorescent dye Fluorescent dye

1999-01-15

2000-10-31

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435968, 435 6, 435 772, 435 78, 435 14, 435 23, 435 24, 536 173, 536 172, C12Q 100, C12Q 168, C12Q 154, C12Q 137

Patent

active

061400416

ABSTRACT:
Fluorescent dyes possess reactive linkers for conjugating to nucleic acids, carbohydrates and peptides. The conjugates fluoresce in the visible and UV spectrum and have an excellent solvochromatic response as compared to other fluorescence or chromatic labels. The conjugates are stable but also have medium sensitive. The fluorescent dyes have little triplet state formation and are not photoreactive, making them an excellent substance for biological investigations. Uses for the dyes include protein labeling, DNA labeling, single molecule spectroscopy and fluorescence. A synthesis of the dyes is disclosed. Methods of use include the detection of carbohydrate-protein interactions.

REFERENCES:
patent: 4140529 (1979-02-01), Pai et al.
patent: 4265900 (1981-05-01), Stolka et al.
patent: 5179457 (1993-01-01), Buchwalter et al.
Shin, et al., Solvatochromatic Behavior of Intramolecular Charge-Transfer Diphenylpolyenes in Homogenous Solution and Microheterogenous J. Phys. Chem. 92: 2945-2956 (1988).
Akiyama, et al., "Direct C.gtorsim.C Triple Bond Formation from the C.dbd.C Double Bond Hydroxylation into the o-Position of a Nitro Group on the Benzene Nucleus with Potassium t-Butoxide in N,N-Dimethylformamide in the Air", Bull. Chem. Soc. Jpn. 68: 2043-2051 (1995).
Roy, et al., "Stereospecific Synthesis of Aryl .beta.-D-N-Acetylglucopyranosides by Phase Transfer Catalysis", Syn. Comm. 20: 2097-2102 (1990).
Eigen, et al., "Sorting single molecules: Application to diagnostics and evolutionary biotechnology", Proc. Natl. Acad. Sci USA 91: 5740-5747 (1994).
Reichardt, "Solvatochromic Dyes as Solvent Polarity Indicators", Chem. Rev. 94: 2319-2358 (1994).
Nie, et al., "Probing Individual Molecules with Confocal Fluorescence Microscopy", Science 266: 1018-1021 (1994).
Rigler, "Fluorescence correlations, single molecule detection and large number screening Applications in biotechnology", J. Biotech. 41: 177-186 (1995).
Goodwin, et al., "Single-Molecular Detection in Liquids by Laser-Induced Fluorescence", Acc. Chem. Res. 29: 607-613 (1996).
Schmidt, et al., "Imaging of single molecule diffusions", Proc. Natl. Acad. Sci. USA 93: 2926-2929 (1996).
Edman, et al., "Conformational transitions monitored for single molecules in solution", Proc. Natl. Acad. Sci. USA 93:6710-6715 (1996).
LaClair, "Analysis of highly disfavored processes through pathway-specific correlated fluorescence", Proc. Natl. Acad. Sci. USA 94: 1623-1628 (1997).
La Clair, "Selective Detection of the Carbohydrate-Bound State of Concanvalin A at the Single Molecule Level", J. Am. Chem. Soc. 119: 7676-7684 (1997).

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