Fluorescent detection method based on enzyme activated conversio

Details Fluorescent detection method based on enzyme activated conversio Fluorescent detection method based on enzyme activated conversio

1987-08-25

1991-05-21

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 25, 435 28, 435 27, 435 71, C12Q 128, C12Q 130, G01N 3353

Patent

active

050174754

ABSTRACT:
Methods are provided for the fluorescent detection of an analyte of interest wherein an amine-substituted, ortho-fused pyrazine fluorophore, with neither nitrogen of the pyrazine ring being fused or substituted, is produced by enzymatic oxidation of a fluorophore precursor substrate which comprises a nitrogen-substituted, cyclic compound. Also provided are novel fluorophore-labelled compounds.

REFERENCES:
patent: 4372745 (1983-02-01), Mandle et al.
Konagaya et al.--Chem. Abst. vol. 100 (1984), p. 47914z.
Lentfer--Chem. Abst. vol. 102 (1985), p. 20790k.
Hofmann et al.--Chem. Abst. vol. 99 (1983), p. 18988g.
Caillaud et al.--Chem. Abst. vol. 99 (1983), p. 84100n.

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