Fluorescent detection method and reagent

Details Fluorescent detection method and reagent Fluorescent detection method and reagent

2001-02-01

2010-02-16

Solola, Taofiq A (Department: 1625)

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

Reexamination Certificate

active

07662973

ABSTRACT:
Disclosed is a method for increasing the fluorescence of a Cyanine dye molecule comprising at least one NO2group characterized by the reduction of the at least one NO2group to NHOH or NH2by the action of a nitroreductase. The cyanine dye molecule comprising at least one NO2group can be used as a substrate for detecting nitroreductase enzyme activity in a composition and allows for the use of a nitroreductase enzyme in an enzyme-reporter system for the detection of analytes, binding reactions or gene expression.

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R. E. Bareiss; J. Kahovec; and P. Kratochvil, “Graphic Representations (Chemical Formulae) of Macromolecules”, Pure and Applied Chemistry, vol. 66, No. 12, pp. 2469-2482 (1994).

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