Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1992-12-21
1994-11-15
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 4, 4351723, 435193, 435968, C12N 910, C12N 1509, C12Q 102, C12Q 148
Patent
active
053647645
ABSTRACT:
Fluorescent compounds useful in the determination of chloramphenicol acetyltransferase (CAT) enzyme activity are described. The compounds are "fluorescent derivatives related in structure to chloramphenicol and are acylated in the presence of CAT to produce fluorescent mono- and diacylated products, which are then physically separated from the reaction mixture and quantitated by means of their fluorescence and/or absorbance. Fluorescent molecules conjugated to chloramphenicol include derivatives of fluorescein, rhodamine, coumarin, dimethylaminonaph-thalene sulfonic acid (dansyl), pyrene, anthracene, nitrobenz-oxadiazole (NBD), acridine and dipyrrometheneboron difluoride.
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Hruby, D. E. et al. BioTechniques 8(2): 170-171 (1990).
Haugland Richard P.
Kang Hee C.
Melner Michael H.
Young Steven L.
Helfenstein Allegra J.
Jacobson Dian C.
Molecular Probes, Inc.
Skaugset Anton E.
Wax Robert A.
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