Fluorescent biological particle detection system

Radiant energy – Means to align or position an object relative to a source or...

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G01N 2164

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058959220

ABSTRACT:
A process and apparatus are provided for detection of viable and potentially hazardous biological particles which may be dispersed in a particulate-containing airstream. The process comprises directing each of the contained particles along a linear path through air, in a sequential manner, and sampling them for determination of their size, whether they are biological and viable, and whether they are present in concentrations greater than background levels. The particle size identifies the particles as respirable or not and the particles are characterized as biological and viable by subjecting each particle in turn, to 340 nm, ultraviolet laser light and looking for the emission of fluorescence which is typically emitted from bacteria or bacterial spore. Fluorescence detected in the 400-540 nm range signals the presence of nicotinamide adenine dinucleotide hydrogen, which is indicative of biological activity or viability. A more compact, and power-saving apparatus results with the preferential use of a solid state, ultraviolet laser, which is actuated only when the particle is passing the laser and only if it is deemed to be a biologically viable candidate.

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