Fluorescent biological particle detection system

Radiant energy – Luminophor irradiation – With ultraviolet source

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G01N 2164

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active

057010129

ABSTRACT:
A process and apparatus are provided for detection of viable and potentially hazardous biological particles which may be dispersed in a particulate-containing airstream. The process comprises directing each of the contained particles along a linear path through air, in a sequential manner, and sampling them for determination of their size, whether they are biological and viable, and whether they are present in concentrations greater than background levels. The particle size identifies the particles as respirable or not and the particles are characterized as biological and viable by subjecting each particle in turn, to 340 nm, ultraviolet laser light and looking for the emission of fluorescence which is typically emitted from bacteria or bacterial spore. Fluorescence detected in the 400-540 nm range signals the presence of nicotinamide adenine dinucleotide hydrogen, which is indicative of biological activity or viability.

REFERENCES:
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Today Science Tomorrow Defence "Detection of Biological Warfare Agents" pp. 11-18, Jim Ho (1994).
Biotechnol. Prog., 1991, 7:21-27 "Monitoring Cell Concentration and Activity by Multiple Excitation Fuorometry" Li et al.
Suffield Memorandum No. 1421 "Detection of BW Agents: Flow Cytometry Measurement of Bacillus Subtillis (BG) Spore Fluorescence", 1993.
Aerosol Science and Technology 4:89-97 (1985) "Performance of a TSI Aerodynamic Particle Sizer", B. T. Chen, Y.S. Chen, H. C. Yeh.
Practical Flow Cytometry, Second Edition, 1988, p. 84, "How a Flow Ctometer Works", A.R. Liss, Inc. NY, NY by Shapiro et al.

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