Fluorescent assays, including immunoassays, with feature of flow

Details Fluorescent assays, including immunoassays, with feature of flow Fluorescent assays, including immunoassays, with feature of flow

1985-09-09

1987-12-29

Marantz, Sidney

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

250302, 2504611, 356 731, 356445, 422 57, 436172, 436501, 436527, 436546, G01N 33533, G01N 33552, G01N 2164, G01N 3500

Patent

active

047161213

ABSTRACT:
An assay method and apparatus employing total internal reflection of excitation radiation at an activated region on the surface of an optically conductive rod or fiber surrounded by a liquid phase of lower index of refraction, so as to excite into fluorescence fluorophores in the liquid present in a thin layer around the rod surface. The induced fluorescence is then gathered and measured. Excitation occurs while flowing a sample of the liquid phase through an enclosure of fixed dimensions bounded in part by the activated region.

REFERENCES:
patent: 4153675 (1979-05-01), Kleinerman
patent: 4293310 (1981-10-01), Weber
patent: 4297273 (1981-10-01), Buckler
patent: 4398894 (1983-08-01), Yamamoto
patent: 4436827 (1984-03-01), Tamagawa
patent: 4447546 (1984-03-01), Hirschfeld
patent: 4558014 (1985-12-01), Hirschfeld
patent: 4582809 (1986-04-01), Block
patent: 4671938 (1987-06-01), Cook

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