Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Reexamination Certificate
2007-07-03
2010-12-07
Tsang, Cecilia (Department: 1654)
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
C530S300000
Reexamination Certificate
active
07847098
ABSTRACT:
The present invention provides a fluorescent amino acid derivative which can be synthesized by simple steps, can be excited particularly by a blue laser ray region of visible light, and has an improved light stability. These objects can be achieved by a fluorescent amino acid derivative which is an acridone derivative substituted with an amino acid to comprise an electrophilic substituent group between the amino acid and the acridone derivative. Instead of a conventional strategy that aminophenylalanine is used as a starting material to form a fluorescent group through a coupling reaction and an intramolecular cyclization reaction, a fluorescent acridone derivative is used as a starting material to furnish the material to a reactive group by a position-specific electrophilic substitution reaction, and then the acridone derivative having the reactive group is allowed to couple with an amino acid derivative.
REFERENCES:
patent: 05-043574 (1993-02-01), None
patent: 2005-287327 (2005-10-01), None
patent: WO 02/099424 (2002-12-01), None
patent: WO 2004/011946 (2004-02-01), None
(http://www.biotech.okayama-u.ac°jp/labs/sisido/sisidol.html.
http://www.biotech.okayama-u.ac.jp/labs/sisido/sisido2.html).
Hamada H, et al, “Position-specific incorporation of a highly photodurable and blue-laser excitable fluorescent amino acid into proteins for fluorescence sensing,” Bioorg Med Chem. May 16, 2005;13(10):3379-84.
Szymaflska et al., “Synthesis of N-[(tert-Butoxy)carbonyl]-3-(9,10-dihydro-9-oxoacridin-2- yl)-L-alanine, a New Fluorescent Aminio Acid Derivative,” Helvetica Chimica Acta, vol. 86, 2003, pp. 3326-3331 Applicants IDS.
Szymańska et al., “Synthesis of N-[(tert-Butoxy)carbonyl]-3-(9,10-dihydro-9-oxoacridin-2-yl)-L-alanine, a New Fluorescent Amino Acid Derivative,” Helvetica Chimica Acta, vol. 86, 2003, pp. 3326-3331.
Sisido Masahiko
Taki Masumi
Heard Thomas S
Kilyk & Bowersox P.L.L.C.
National University Corporation Okayama University
Tsang Cecilia
LandOfFree
Fluorescent amino acid derivative and production method of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Fluorescent amino acid derivative and production method of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescent amino acid derivative and production method of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4227216