Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Reexamination Certificate
2007-06-12
2007-06-12
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
C435S257100
Reexamination Certificate
active
10511929
ABSTRACT:
In situ fluorescence method to monitor state of sulfur-deprived algal culture's ability to produce H2under sulfur depletion, comprising: a) providing sulfur-deprived algal culture; b) illuminating culture; c) measuring onset of H2percentage in produced gas phase at multiple times to ascertain point immediately after anerobiosis to obtain H2data as function of time; and d) determining any abrupt change in three in situ fluorescence parameters; i) increase in Ft(steady-state level of chlorophyll fluorescence in light adapted cells); ii) decrease in Fm′, (maximal saturating light induced fluorescence level in light adapted cells); and iii) decrease in ΔF/Fm′=(Fm′−Ft)/Fm′ (calculated photochemical activity of photosystem II (PSII) signaling full reduction of plastoquinone pool between PSII and PSI, which indicates start of anaerobic conditions that induces synthesis of hydrogenase enzyme for subsequent H2production that signal oxidation of plastoquinone pool asmain factor to regulate H2under sulfur depletion.
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Makarova Valeriya
Rubin Andrew B.
Seibert Michael
Tsygankov Anatoly A.
Gitomer Ralph
Midwest Research Institute
White Paul J.
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