Fluorescence spectroscopy assay means with fluorescent chelate o

Details Fluorescence spectroscopy assay means with fluorescent chelate o Fluorescence spectroscopy assay means with fluorescent chelate o

1980-03-07

1983-02-15

Fagelson, Anna P.

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

250305, 2504612, 435 7, 435 35, 436800, 436547, 424 71, G01N 2133, G01N 3348, G01N 3352

Patent

active

043741207

ABSTRACT:
An improved method of determining the nature of a substance by fluoroscence spectroscopy wherein a fluorescent marker is coupled to the molecules of the substance comprises the use of a marker having a longer period of fluorescence than those of possible sources of noise and by employing an exciting radiation pulse of short duration so that the fluorescence of the marker is detected after the objectionable sources of fluorescence have ceased; the marker including a fluorescent lanthanide chelate complex.

REFERENCES:
patent: 4058732 (1977-11-01), Wieder
patent: 4133873 (1979-01-01), Noller
patent: 4259313 (1981-03-01), Frank
Zheltvai et al., Koordinatsionnaia Khimiia, vol. 2, 1976, pp. 1600-1608.
Moller et al., Chem. Rev., vol. 65, 1965, p. 1.
Makhijani et al., J. Ind. Chem. Soc., vol. 60, 1978, p. 840.
Nakatani et al., Rev. Phys. Chem., Japan, vol. 42, 1972, p. 103.
Polmektov et al., Zh. Neorg. Khim, vol. 19, 1974, p. 3257.
Wieder, Chem Abs, vol. 90, 1979 (citing Immunoflouresc Tech. Proc. Int. Conf. 6th, 1978, p. 67-80).
Leung, Biochem & Biophys. Res. Comm. vol. 75, No. 1, 1977, pp. 149-155.

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