Fluorescence spectrometry employing excitation of bleaching inte

Details Fluorescence spectrometry employing excitation of bleaching inte Fluorescence spectrometry employing excitation of bleaching inte

1975-11-18

1977-04-19

McGraw, Vincent P.

Optics: measuring and testing

Range or remote distance finding

With photodetection

250459, 356 98, G01J 330

Patent

active

040185303

ABSTRACT:
An improved system is desired for examining fluorescent material. The method comprises the steps of illuminating material with radiation at a fluorescent excitation wavelength of said material and at an intensity sufficient to cause bleaching of the material; detecting over a time interval commencing with initial illumination of the material, fluorescent emission produced by the material during bleaching of the latter by the radiation; and integrating over the interval the fluorescent emission detected during the bleaching of the material. Means are provided for illuminating the material to cause bleaching, for detecting fluorescent emission produced during bleaching, for measuring the decay time interval, and for integrating a signal from the detecting means over a time interval.

REFERENCES:
patent: 3829696 (1974-08-01), Birnbaum
patent: 3886363 (1975-05-01), Ohnishi et al.
Microscope Phase Fluorometer for Determining the Fluorescence Lifetimes of Fluorochromes; Venetta; Review of Sci. Inst.; June 1959, pp. 450-451.

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