Fluorescence resonance energy transfer analyzer

Details Fluorescence resonance energy transfer analyzer Fluorescence resonance energy transfer analyzer

2004-01-09

2008-11-11

Siefke, Sam P (Department: 1797)

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Means for analyzing liquid or solid sample

C422S068100, C422S082050, C422S082090, C436S164000, C436S172000

Reexamination Certificate

active

07449151

ABSTRACT:
An analyzer for measuring an FRET (fluorescence resonance energy transfer) efficiency of a specimen containing a donor and an acceptor. The analyzer has an illuminator, an optical system, a detector and a calculator. The illuminator emits light for donor excitation and acceptor bleaching. The detector detects fluorescence from the specimen. The calculator calculates the FRET efficiency using the output of the detector. The detector independently detects the fluorescence in wavelength regions. One of the regions has a larger overlap with the fluorescence spectrum of the acceptor than with that of the donor.

REFERENCES:
patent: 4900934 (1990-02-01), Peeters et al.
patent: 5866355 (1999-02-01), Miyakawa et al.
patent: 6396053 (2002-05-01), Yokoi
patent: 2001/0012152 (2001-08-01), Satou
patent: 2002/0085293 (2002-07-01), Stuckey
Miyawaki et al.; “Fluorescent Indicators for Ca2+Based on Green Fluorescent Proteins and Calmodulin”; Nature, vol. 388, (Aug. 1997); pp. 882-887.
Sawano et al.; “Multicolor Imaging for Ca2+and Protein Kinase C Signals Using Novel Epifluorescence Microscopy”; Biophysical Journal; vol. 82; (Feb. 2002); pp. 1076-1085.
Miyawaki et al.; “Monitoring Protein Conformations and Interactions by Fluorescence Resonance Energy Transfer Between Mutants of Green Fluorescent Protein”; Methods in Enzymology, vol. 327; (2000); pp. 472-501.
Wouters et al.; “FRET Microscopy Demonstrates Molecular Association of Non-Specific Lipid Transfer Protein (nsL-TP) With Fatty Acid Oxidation Enzymes in Peroxisomes”; The EMBO Journal; vol. 17; No. 24; pp. 7179-7189; (1998).
Bastiaens et al.; “Imaging the Molecular State of Proteins in Cells by Fluorescence Resonance Energy Transfer (FRET) Sequential Photobleaching of Forster Donor-Acceptor Pairs”; Cell-Cell Communication; pp. 77-83; (1995).
HPK Catalog; Aqua Cosmos/Ashura (2003).
Miyawaki; “FRET Between Molecules With the Use of GFP”; Seitai no Kagaku; vol. 53, No. 1; (2002); pp. 75-81; w/ English Translation.
Sawano et al., “Multicolor Imaging of Ca2+and Protein Kinase C Signals Using Novel Epifluorescence Microscopy”, Biophysical Journal, vol. 82, Feb. 2002, pp. 1076-1085.
Miyawaki et al., “Monitoring Protein Conformations and Interactions by Fluorescence Resonance Energy Transfer Between Mutants of Green Fluorescent Protein”, Methods in Enzymology, vol. 327, pp. 472-500.

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