Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1976-10-12
1979-11-13
Fagelson, Anna P.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230R, 23230B, 435 7, 250302, 260112R, 260112B, 424 1, 424 7, 424 11, 424 12, 424 13, G01N 2138, G01N 3100, G01N 3316, C07G 700
Patent
active
041743849
ABSTRACT:
Immunoassays are provided employing antibodies and a fluorescer-quencher (F-Q) chromophoric pair, wherein one or both of the chromophoric pair are bonded to antibodies. Depending on the particular ligand of interest, various reagent combinations can be employed, where the amount of quenching is directly related to the amount of ligand present in the assay medium.
In carrying out the assay, the unknown and antibody specific for the ligand of interest to which is bound one of the F-Q pair, are combined in an aqueous buffered medium. Depending on the protocol, different assay reagents are employed in the aqueous buffered medium: (1) ligand analog bonded to the other of the F-Q pair; (2) antibodies specific for the ligand to which is bound the other of the F-Q pair or; finally, (3) a combination of a plurality of ligands bonded together through linking groups to a hub molecule, usually a polymer, in combination with antibody bound to the other of the F-Q pair. The composition is irradiated with light at a wavelength, absorbed by the fluorescing molecule and the amount of fluorescence determined. By employing appropriate standards, the presence and amount of the ligand can be determined.
REFERENCES:
patent: 3992631 (1976-11-01), Harte
patent: 3996345 (1976-12-01), Ullman
patent: 3998943 (1976-12-01), Ullman
Winkler, Biochemistry, vol. 8, Jun. 1969, pp. 2586-2590.
Wachsmith, Histochem., vol. 38, No. 4, 1974, pp. 339-350.
Cohen, The J. of Immunology, vol. 98, 1967, pp. 143-149.
Kabat, Exptl. Immunochem., C. C. Thomas, Springfield, Ill., 2nd ed., 1961, pp. 809-811.
Williams, Methods in Immunol. & Immunochem., Academic Press, N.Y., vol. III, 1971, pp. 435-453.
Schwarzberg Moshe
Ullman Edwin F.
Fagelson Anna P.
Rowland Bertram I.
Syva Company
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