Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1977-02-07
1980-04-22
Marantz, Sidney
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 23915, 424 12, G01N 3316
Patent
active
041995597
ABSTRACT:
Immunoassays are provided employing antibodies and a fluorescer-quencher (F-Q) chromophoric pair, wherein one or both of the chromophoric pair are bonded to antibodies. Depending on the particular ligand of interest, or whether antibodies are to be assayed, various reagent combinations can be employed, where the amount of quenching is directly related to the amount of ligand or antibody present in the assay medium.
In carrying out the assay for ligands, the unknown and antiody specific for the ligand of interest to which is bound one of the F-Q pair, are combined in an aqueous buffered medium. Depending on the protocol, different assay reagents are employed in the aqueous buffered medium: (1) ligand analog bonded to the other of the F-Q pair; (2) antiobodies specific for the ligand to which is bound the other of the F-Q pair or; finally, (3) a combination of a plurality of ligands bonded together through linking groups to a hub molecule, usually a polymer, in combination with antibody bound to the other of the F-Q pair. The fluorescar is electronically excited, for example by irradiation with light at a wavelength, absorbed by the fluorescing molecule and the amount of emitted light determined. By employing appropriate standards, the presence and amount of the ligand can be determined.
The technique for the determination of antibodies in a competitive mode is substantially the same except that ligand is added as a reagent.
REFERENCES:
patent: 3899298 (1975-08-01), Szczesniak
patent: 3996345 (1976-12-01), Ullman
Schwarzberg Moshe
Ullman Edwin F.
Marantz Sidney
Rowland Bertram I.
Syva Company
LandOfFree
Fluorescence quenching with immunological pairs in immunoassays does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Fluorescence quenching with immunological pairs in immunoassays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescence quenching with immunological pairs in immunoassays will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1475350