Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Signal modification or steric inhibition
Patent
1991-03-25
1993-04-27
Hill, Jr., Robert J.
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
Signal modification or steric inhibition
364497, 435973, 436805, 436815, 436816, 436817, G01N 33542
Patent
active
052061795
ABSTRACT:
A methodology is presented which relates in general to fluorescence polarization immunoassays (FPIA) and modifications of current processes wherein detection and quantification of several commonly abused or therapeutic drugs in a single biological fluid sample are determined utilizing manual or current software or related equipment to allow the sequential and simultaneous performance of more than one FPIA assay. The methodology involves combining the reagents either separately or pre-mixed, for multiple assays in a single reagent package, these reagents being used to assay quantitative amounts of each of the assay analytes in a sequential step manner. The assay being performed by mixing the sample with a combination reagent and then initiating a specific reaction for each of the separate analytes by sequentially adding a specific reagent, i.e. tracer for each and reading the results during each separate stage to determine the specific reaction taken place and utilization of manual or software modification monitoring to subtract out the contribution to total signal of each specific reagent added previously to the stage of interest.
REFERENCES:
patent: 4778751 (1988-10-01), El Shami et al.
patent: 4863876 (1989-09-01), Hevey
David B. Gordon, "Measurement of Angiotensinogen in Human Serum by Fluorescence Polarization Immunossay" Clin. and Exper. Hyper. Theory and Practice, A10(3), 485-503 (1988).
D. L. Colbert and M. Childerstone, "Multiple Drugs of Abuse in Urine Detected with a Single Reagent and Fluorescence Polarization"-Clin. Chem. 33/10 1921-1923 (1987).
Frank V. Bright and Linda B. McGown, "Homogeneous Immunoassay of Phenobarbital by Phase-Resolved Fluorescence Spectroscopy"-Talanta, vol. 32, No. 1, pp. 15-18, 1985.
Mohammad H. H. Al-Hakiem, D. S. Smith and J. Landon, "Development of Fluoroimmunoassay for the Determination of Individual or Combined Levels of Procainamide and N-Acetylprocainamide in Serum"-Journal of Immunoassay, 3(1), 91-110 (1982).
A. T. M. Al-Ani, M. H. H. Al-Hakiem and T. Chard, "Sequential Fluoroimmunoassay for Measurement of Pregnancy Specific B, Glycoprotein Using Antibody Coupled to Magnetisable Particles"-Clinica Chemica Acta, 112 (1981) 91-98.
Abbott Laboratories
Breininger Thomas M.
Hill Jr. Robert J.
LandOfFree
Fluorescence polarization immunoassays for multiple analytes usi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Fluorescence polarization immunoassays for multiple analytes usi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescence polarization immunoassays for multiple analytes usi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2326736