Fluorescence polarization immunoassay utilizing substituted carb

Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase

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436528, 436537, 436800, G01N 33533, G01N 33536

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active

046686401

ABSTRACT:
This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescence polarization techniques to provide a means for determining the amount of a specific ligand present in a sample.

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Chen, Analytical Letters, 10(10), 787-795 (1977).
Dandliker et al., Immunochemistry, vol. 4, 1967, pp. 295-302.

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