Fluorescence polarization immunoassay

Details Fluorescence polarization immunoassay Fluorescence polarization immunoassay

1998-05-21

2000-05-23

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Signal modification or steric inhibition

436546, 436815, 436816, 436825, G01N 33542, G01N 33533

Patent

active

06066505&

ABSTRACT:
In a fluorescence polarization assay for determining the amount of a target analyte in a test sample, wherein the amount of analyte is related to the amount of fluorescence emitted from the analyte-containing reagent medium, the improvement comprising contacting the analyte-containing reagent medium with of at least one compound from the group consisting of 1,10-phenanthroline, 8-hydroxy-7-iodo-5-quinoline, naphthalene-1-sulfonic acid, salts thereof, and any combination thereof.

REFERENCES:
patent: 4252783 (1981-02-01), Kam et al.
patent: 4492762 (1985-01-01), Wang et al.
The Merck Index of Chemicals and Drugs, Seventh Edition. Merck & Co., Inc. NJ, USA, p. 791, 1960.
Dandliker, W. B. and Feigen, G. A., Quantification of the Antigen-Antibody Reaction by the Polarization of Fluorescence, 1961, vol. 5, No. 4, 299-304 .

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