Fluorescence polarization-based homogenous assay for aflatoxins

Details Fluorescence polarization-based homogenous assay for aflatoxins Fluorescence polarization-based homogenous assay for aflatoxins

2008-05-27

2010-10-12

Yu, Melanie (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007920, C435S007930, C435S007940, C436S164000, C436S172000, C436S501000

Reexamination Certificate

active

07811775

ABSTRACT:
A homogeneous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.

REFERENCES:
patent: 4444879 (1984-04-01), Foster et al.
patent: 4772551 (1988-09-01), Hart et al.
patent: 4835100 (1989-05-01), Dixon et al.
patent: 5166078 (1992-11-01), McMahon et al.
patent: 5171686 (1992-12-01), Cotty
patent: 5262333 (1993-11-01), Heiman et al.
patent: 5380825 (1995-01-01), Stenglein et al.
patent: 5420016 (1995-05-01), Boguslaski et al.
patent: 5427960 (1995-06-01), Wang et al.
patent: 5741654 (1998-04-01), Michel et al.
patent: 5976820 (1999-11-01), Jolley et al.
patent: 6432632 (2002-08-01), Nakayama et al.
patent: 6482601 (2002-11-01), Nasir et al.
patent: 6812036 (2004-11-01), Nasir et al.
patent: 2002/0055180 (2002-05-01), Nasir et al.
patent: 2002/0110803 (2002-08-01), Dhar et al.
patent: 97/19950 (1997-06-01), None
Nasir et al., Fluorescence Polarization: An Anallytical Tool for Immunoassay and Drug Discovery, Combinatorial Chemistry & High Throughput Screening, 1999, 2, pp. 177-190.
Richard et al., Analysis of Naturally Occurring Mycotoxing in Feedstuffs and Food, J. Anim. Sci. 1993 71: pp. 2563-2574.
Holladay, Steven David, “Production of specific antibody against aflatoxin M1,” Dissertation Abstracts International, vol. 50, No. 10, p. 4298-B (Apr. 1990).
Pestka, et al., “Immunological Assays for Mycotoxin Detection,” Food Technology, vol. 49, No. 2, pp. 120-128 (Feb. 1995).
Neely, et al., “Spectral Studies on the Deoxyribonucleic Acid-Aflatoxin B1 System,” Biochemistry, vol. 9, pp. 1862-1866 (1970).
Fukal, et al., “Choice of Procedure Conditions for Radioimmunoassay of Aflatoxin,” J. Radioanal. Nucl. Chem. Letters, vol. 108, pp. 259-268 (1986).
Holladay, et al., “Evaluation of an indirect enzyme-linked immunosorbent assay for screening antibody against aflatoxins,” Am J Vet Res, vol. 52, No. 2, pp. 222-223 (Feb. 1991).
Maragos, et al., “Fluorescence Polarization as a Means for Determination of Fumonisins in Maize,” J. Agric. Food Chem., vol. 49, pp. 596-602 (Feb. 2001).
Nasir et al., “Fluorescence Polarization: An Analytical Tool for Immunoassay and Drug Discovery”, Combinatorial Chemistry & High Throughput Screening, vol. 2, pp. 177-190 (1999).
Nasir et al., “Development of a Fluorescence Polarization Assay for the Determination of Aflatoxins in Grains,” J. Agric. Food Chem., vol. 50, pp. 3116-3121 (2002).
Langone, J., et al., “Aflatoxin B1: Specific Antibodies and Their-Use in Radioimmunoassay,” Journal of the American Cancer Institute, vol. 56, No. 3, pp. 591-595 (Mar. 1976).
Sizaret, P., et al., “Detection of Aflatoxins and Related Metabolites by Radioimmunoassay,” JNCI, vol. 69, No. 6, pp. 1375-1381 (Dec. 1982).
Whitaker, et al., “Variability Associated with Analytical Methods Used to Measure Aflatoxin in Agricultural Commodities,” Journal of AOAC International, vol. 79, No. 2, pp. 476-485 (1996).
Wilson, T., et al., “Use of the Mycosep Multifunctional Cleanup Column for Liquid Chromatographic Determination of Aflatoxins in Agricultural Products,” J. Assoc. Off. Anal. Chem., vol. 74, No. 6, pp. 951-956 (1991).
Newberne, P., et al., “Acute and Chronic Effects of Aflatoxin on the Liver of Domestic and Laboratory Animals: A Review,” Cancer Research, vol. 29, pp. 236-250 (Jan. 1969).
Trucksess, M., et al., “Immunoaffinity Column Coupled With Solution Fluorometry or Liquid Chromatography Postcolumn Derivatization for Determination of Aflatoxins in Corn, Peanuts, and Peanut Butter: Collaborative Study,” J. Assoc. Off. Anal. Chem., vol. 74, No. 1, pp. 81-88 (1991).
Wild, C., et al., “Aflatoxin Detected in Human Breast Milk by Immunoassay,” Int. J. Cancer, vol. 40, pp. 328-333 (1987).
Trucksess, et al., “Multifunctional Column Coupled with Liquid Chromatography for Determination of Aflatoxins B1, B2, G1, and G2 in Corn, Almonds, Brazil Nuts, Peanuts, and Pistachio Nuts: Collaborative Study,” Journal of AOAC International, vol. 77, No. 6, pp. 1512-1521 (1994).
Campbell, T., et al., “Implication of Mycotoxins for Human Health,” J. Agr. Food Chem., vol. 22, No. 6, pp. 1006-1015 (1974) .
Seitz, L., “Comparison of Methods for Aflatoxin Analysis by High-Pressure Liquid Chromatography,” Journal of Chromatography, vol. 104, pp. 81-89 (1975).
Asao, T., et al., “The Structures of Aflatoxins B and G1,” Journal of the American Chemical Society, vol. 87, No. 4, pp. 882-886 (Feb. 20, 1965).
Maragos, C., et al., “Fiber-optic Immunosensor for Mycotoxins,” Natural Toxins, vol. 7, pp. 371-376 (1999).
Holladay, S.D., “Preparation of Protein Conjugatable Aflatoxin Oxime Derivative from Significantly Reduced Starting Quantities,” Veterinary Human Toxicology, vol. 31, No. 6, pp. 526-527 (1989).

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