Fluorescence polarization assay

Details Fluorescence polarization assay Fluorescence polarization assay

2007-05-15

2007-05-15

Le, Long V. (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S004000, C436S523000, C436S164000

Reexamination Certificate

active

10628879

ABSTRACT:
The present invention relates to methods for detecting the presence of one or more analytes of interest in a sample by measuring changes in fluorescence anisotropy as a result of binding of the analytes to specific aptamers. The aptamers are immobilized on a solid support and may be in the form of an array.

REFERENCES:
patent: 5037207 (1991-08-01), Tomei et al.
patent: 5405783 (1995-04-01), Pirrung et al.
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5445935 (1995-08-01), Royer
patent: 5582981 (1996-12-01), Toole et al.
patent: 5631169 (1997-05-01), Lakowicz et al.
patent: 5641629 (1997-06-01), Pitner et al.
patent: 5641633 (1997-06-01), Linn et al.
patent: 5744305 (1998-04-01), Fodor et al.
patent: 5756292 (1998-05-01), Royer
patent: 5792613 (1998-08-01), Schmidt et al.
patent: 5800989 (1998-09-01), Linn et al.
patent: 5840867 (1998-11-01), Toole et al.
patent: 5989823 (1999-11-01), Jayasena et al.
patent: 6074609 (2000-06-01), Gavin et al.
patent: 6166804 (2000-12-01), Potyrailo et al.
patent: 6172218 (2001-01-01), Brenner
patent: 6242246 (2001-06-01), Gold et al.
patent: 6261783 (2001-07-01), Jayasena et al.
patent: 6297018 (2001-10-01), French et al.
patent: 6309701 (2001-10-01), Barbera-Guillem
patent: 6327410 (2001-12-01), Walt et al.
patent: 6365418 (2002-04-01), Wagner et al.
patent: 6399302 (2002-06-01), Lannigan et al.
patent: 6423493 (2002-07-01), Gorenstein et al.
patent: 6544776 (2003-04-01), Gold et al.
patent: 6573045 (2003-06-01), Karn et al.
patent: 6576419 (2003-06-01), Wei et al.
patent: 6610504 (2003-08-01), Yuan
patent: 6680377 (2004-01-01), Stanton et al.
patent: 2001/0033374 (2001-10-01), Hoyt
patent: 2002/0037506 (2002-03-01), Lin et al.
patent: 2002/0061531 (2002-05-01), Xiao-Chun
patent: WO 00/61803 (2000-10-01), None
patent: WO 00/70329 (2000-11-01), None
patent: WO 01/57259 (2001-08-01), None
patent: WO 02/010750 (2002-02-01), None
patent: WO 02/24959 (2002-03-01), None
patent: WO 02/030561 (2002-04-01), None
Spridonova et al, DNA aptamers as radically new recognition elements for biosensors, Jun. 2002, Biochem, 67, 706-709.
Fang et al, Molecular aptamer for real-time oncoprotein platelet derived growth factor monitoring by fluorescence anisotropy, 2001, 73, 5752-5757.
Hesselberth et al, In vivo selection of nucleic acids for diagnostic applications, 2000, Rev Mol Biotech, 74, 15-25.
Lee et al, A fiber-optic microarray biosensor using aptamers as receptors, 2000, Anal Biochem, 282:142-146.
Lakowicz et al, Anisotropy-based sensing with reference fluorophores, 1998, Anal Biochem, 267, 397-405.
Sevenich et al., “DNA binding and oligomerization of NtrC studied by fluorescence anisotropy and fluorescence correlation spectroscopy,” Nucleic Acids Research, 1998, vol. 26, No. 6, pp. 1373-1381.
Hesselberth et al., “In vitro selection of nucleic acids for diagnostic applications,” Reviews in Molecular Biotechnology 74 (2000) 15-25, Elsevier.
Potyrailo et al., “Adapting Selected Nucleic Acid Ligands (Aptamers) to Biosensors,” Anal. Chem. 1998, 70, pp. 3419-3425.
Chinnapen et al, “Hemin-Stimulated Docking of Cytochrome c to a Hemin-DNA Aptamer Complex,” Biochemistry 2002, 41, pp. 5202-5212.
Yeakley et al., “Profiling alternative splicing on fiber-optic arrays,” Nature Biotechnology, Apr. 2002, vol. 20, pp. 353-358.
Zhou H, et al., “Solution and chip arrays in protein profiling.” Trends in Biotechnology Oct. 19, 2001(10 Suppl):S34-9.
Jhaveri et al. “In Vitro Selection of Phosphorothiolated Aptamers” Nature Biotechnology, Dec. 2000, 18(12): 1293-1297.
Supplementary European Search Report, European Patent No. 03766936.3, European Patent Office. Jan. 22, 2007, 4 pages.

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